Alcohol and Health: Standards of Consumption, Benefits and Harm – a Review (2019)
- Authors:
- Autor USP: SILVA, JOÃO BATISTA DE ALMEIDA E - EEL
- Unidade: EEL
- DOI: 10.17221/117/2018-CJFS
- Subjects: ABUSO DE ÁLCOOL; BEBIDAS ALCOÓLICAS
- Keywords: moderate drinking; human health; patterns of consumption; standard dose
- Language: Inglês
- Abstract: The research on alternative solvents and cosolvents is relevant when envisioning the improvement of biocatalytic reactions. Among these solvents and cosolvents, deep eutectic solvents (DES) may be considered as customizable new reaction media for biocatalysis. Accordingly, in this work, 16 DES aqueous solutions, as well as the individual DES components at the same conditions, have been investigated in laccase-catalyzed reactions. Cholinium- and betaine-based DES formed with polyols at different molar ratios and concentrations were evaluated. The results reported show that in the presence of most DES the laccase activity is preserved and, with a particular DES, enhanced up to 200%. Molecular docking studies demonstrated that while most DES components establish hydrogen bonds with the enzyme amino acids, those that establish stronger interactions with the enzyme (expressed by absolute values of docking affinity energies) lead to an enhanced laccase activity. Finally, the laccase stability was evaluated in additional tests under extreme storage temperatures (60 °C and −80 °C). Although no significant protection to high temperatures was afforded by DES, an enhanced laccase activity when stored at low temperatures was found, at least up to 20 days. Combining experimental results and molecular docking, this work shows that DES can be designed as cosolvents to improve biocatalytic reactions.
- Imprenta:
- Source:
- Título: Czech journal of food sciences
- ISSN: 12121800
- Volume/Número/Paginação/Ano: v.36, n.6, p.427-440, 2019
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by-nc
-
ABNT
VIEIRA, Isabela Maria Monteiro et al. Alcohol and Health: Standards of Consumption, Benefits and Harm – a Review. Czech journal of food sciences, v. 36, n. 6, p. 427-440, 2019Tradução . . Disponível em: https://doi.org/10.17221/117/2018-CJFS. Acesso em: 28 dez. 2025. -
APA
Vieira, I. M. M., Santos, B. L. P., Ruzene, D. dos S., Branyik, T., Teixeira, J. A., Silva, J. B. A. e, & Silva, D. P. da. (2019). Alcohol and Health: Standards of Consumption, Benefits and Harm – a Review. Czech journal of food sciences, 36( 6), 427-440. doi:10.17221/117/2018-CJFS -
NLM
Vieira IMM, Santos BLP, Ruzene D dos S, Branyik T, Teixeira JA, Silva JBA e, Silva DP da. Alcohol and Health: Standards of Consumption, Benefits and Harm – a Review [Internet]. Czech journal of food sciences. 2019 ;36( 6): 427-440.[citado 2025 dez. 28 ] Available from: https://doi.org/10.17221/117/2018-CJFS -
Vancouver
Vieira IMM, Santos BLP, Ruzene D dos S, Branyik T, Teixeira JA, Silva JBA e, Silva DP da. Alcohol and Health: Standards of Consumption, Benefits and Harm – a Review [Internet]. Czech journal of food sciences. 2019 ;36( 6): 427-440.[citado 2025 dez. 28 ] Available from: https://doi.org/10.17221/117/2018-CJFS - Brewery waste reuse for protease production by lactic acid fermentation
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Informações sobre o DOI: 10.17221/117/2018-CJFS (Fonte: oaDOI API)
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