Pulmonary paracoccidioidomycosis (PCM) in IL-10 knock-out (KO) mice (2005)
- Authors:
- Autor USP: CALICH, VERA LUCIA GARCIA - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Objective: Studies in experimental PCM have demonstrated that immunological resistance is linked to a preferential Th1 immune response whereas susceptibility is associated with absence or low levels of IFN-gamma. Patients with the severe and mild forms of the disease as well as susceptible and resistant mice to PCM secrete IL-10 throughout the course of the disease but its precocious or enhanced synthesis appear to be associated with the most severe aspects of the infection. To better understand the in vivo role of IL-10, pulmonary PCM was comparatively studied in IL-10 KO mice and their normal counterparts of the C57BL/6 strain. Methods and Results: Wild type (WT) (n=5-8) and IL-10 KO male mice (n=5-8) were i.t. infected with 1x106 yeast cells of P. brasiliensis. Mice were sacrificed 8 weeks after infection and the severity of disease determined by organ CFU counts and lungs histopathology. Pulmonary cytokines and levels of specific isotypes were evaluated by ELISA in lung homogenates or serum samples, respectively. At 8 week after infection, IL-10 KO mice presented decreased fungal loads in the lungs (KO 3.43±0.18; WT 5.15±0.15 log10) and no fungal cells were detected in the livers and spleens. Lung histology revealed the presence of numerous granulomatous lesions in control mice whereas KO animals presented almost no fungal cells and lesions indicating an evident tendency to cure. The much less severe disease of IL-10 KO mice was accompanied by lowlevels of IgG2a (KO 2.24±1.10; WT 10.69±0.53 log2), IgG2b (KO 6.57±0.41; WT 10.75±0.68 log2) and IgG3 (KO 1.99±0.97; WT 5.32±0 log2) and decreased amounts of pulmonary IL-12 (KO 167.25±34; WT 305.63±41 pg/ml). Conclusion: Genetically determined deficiency of IL-10 results in a less severe PCM which appears to evolve to microbiological cure. Interestingly, this picture was not concomitant with a decreased Th2 immunity but was associated with a diminished Th1 immunity
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
COSTA, Tânia Alves da e CALICH, Vera Lúcia Garcia. Pulmonary paracoccidioidomycosis (PCM) in IL-10 knock-out (KO) mice. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 17 mar. 2026. -
APA
Costa, T. A. da, & Calich, V. L. G. (2005). Pulmonary paracoccidioidomycosis (PCM) in IL-10 knock-out (KO) mice. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Costa TA da, Calich VLG. Pulmonary paracoccidioidomycosis (PCM) in IL-10 knock-out (KO) mice. Resumos. 2005 ;[citado 2026 mar. 17 ] -
Vancouver
Costa TA da, Calich VLG. Pulmonary paracoccidioidomycosis (PCM) in IL-10 knock-out (KO) mice. Resumos. 2005 ;[citado 2026 mar. 17 ] - cd28 costimulatory molecule deficiency in more severe paracoccidioides brasiliensis infection but protects mice from life-threatening immunopathology
- Paracoccidioides brasiliensis infection determines dentric cells to diferentiate to the plasmacytoid subpopulation which induces a more severe pulmonary infection when transfered to resistant mice
- The IDO-AhR axis controls Th17/Treg immunity in a pulmonary model of fungal infection
- TLR-2 is a negative regulator of TH17 cells and tissue pathology in a pulmonary model of fungal infection
- Absence of TLR2 results in less severe paracoccidioidomycosis but increased inflammatory response caused by PMN & TH17 cells
- Toll like receptors and the adaptor molecule MYD88 play an important role in pulmonary paracoccidioidomycosis
- TLR2 is a negative regulator of Th17 cells and tissue pathology in a pulmonary model of fungal infection
- Human alfa / beta and gama / delta t cells respond to proteins of the yeast form of paracoccidioides brasiliensis
- The immune response of high (H) and low responders (L) mice (selection III) and F1 (HIII x LIII) hybrids to paracoccidioides brasiliensis infection
- PMN leukocytes play a more prominent role in natural immunity of susceptible than resistant mice to pulmonary paracoccidiodomycosis
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