An ascomycota coculture in batch bioreactor is better than polycultures for cellulase production (2018)
- Authors:
- Autor USP: MILAGRES, ADRIANE MARIA FERREIRA - EEL
- Unidade: EEL
- DOI: 10.1007/s12223-018-0588-1
- Assunto: BIOTECNOLOGIA
- Agências de fomento:
- Language: Inglês
- Abstract: Efficient hydrolysis of holocellulose depends on a proper balance between cellulase (endoglucanase, exoglucanase, β-glucosidase) and xylanase activities. The present study aimed to induce the production of cellulases and xylanases using liquid cultures (one, two, three, and four fungal strains on the same bioreactor) of wild strains of Trichoderma harzianum, Aspergillus niger, and Fusarium oxysporum. The strains were identified by amplification and analysis of the ITS rDNA region and the obtained sequences were deposited in Genbank. Enzymes (endoglucanase, exoglucansae, β-glucosidase, and xylanase activities) and the profile of extracellular protein isoforms (SDS-PAGE) produced by different fungal combinations (N = 14) were analyzed by Pearson’s correlation matrix and principal component analysis (PCA). According to our results, induction of endoglucanase (19.02%) and β-glucosidase (6.35%) were obtained after 4 days when A. niger and F. oxysporum were cocultured. The combination of A. niger–T. harzianum produced higher endoglucanase in a shorter time than monocultures. On the contrary, when more than two strains were cultured in the same reactor, the relationships of competition were established, trending to diminish the amount of enzymes and the extracellular protein isoforms produced. The xylanase production was sensible to stress produced by mixed cultures, decreasing their activity. This is important when the aim is to produce cellulase-free xylanase. In addition, exoglucanase activity did not change in the combinations tested.M13 A02
- Imprenta:
- Source:
- Título: Folia Microbiologica
- ISSN: 0015-5632
- Volume/Número/Paginação/Ano: v.63, p.467-478, 2018
- Este periódico é de acesso aberto
- Este artigo NÃO é de acesso aberto
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ABNT
HERNÁNDEZ, Christian et al. An ascomycota coculture in batch bioreactor is better than polycultures for cellulase production. Folia Microbiologica, v. 63, p. 467-478, 2018Tradução . . Disponível em: https://doi.org/10.1007/s12223-018-0588-1. Acesso em: 11 fev. 2026. -
APA
Hernández, C., Milagres, A. M. F., Marrufo, G. V., Páz, K. M. M., Pérez, J. A. G., & Alarcón, E. (2018). An ascomycota coculture in batch bioreactor is better than polycultures for cellulase production. Folia Microbiologica, 63, 467-478. doi:10.1007/s12223-018-0588-1 -
NLM
Hernández C, Milagres AMF, Marrufo GV, Páz KMM, Pérez JAG, Alarcón E. An ascomycota coculture in batch bioreactor is better than polycultures for cellulase production [Internet]. Folia Microbiologica. 2018 ;63 467-478.[citado 2026 fev. 11 ] Available from: https://doi.org/10.1007/s12223-018-0588-1 -
Vancouver
Hernández C, Milagres AMF, Marrufo GV, Páz KMM, Pérez JAG, Alarcón E. An ascomycota coculture in batch bioreactor is better than polycultures for cellulase production [Internet]. Folia Microbiologica. 2018 ;63 467-478.[citado 2026 fev. 11 ] Available from: https://doi.org/10.1007/s12223-018-0588-1 - A Chrysoporthe cubensis enzyme cocktail produced from a low-cost carbon source with high biomass hydrolysis efficiency
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Informações sobre o DOI: 10.1007/s12223-018-0588-1 (Fonte: oaDOI API)
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