In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse (2019)
- Authors:
- USP affiliated authors: MILAGRES, ADRIANE MARIA FERREIRA - EEL ; FERRAZ, ANDRÉ LUIS - EEL
- Unidade: EEL
- Assunto: BIOTECNOLOGIA
- Keywords: Sugarcane bagasse; Bacillus pumilus; Xylanase; Xylan extraction; Xylooligosaccharides; Luminase
- Language: Inglês
- Abstract: The purpose of this work was to investigate the enzymatic extraction of xylan using xylanases active in alkaline conditions. One on-site produced and three commercial xylanases designed to boost bleaching of pulped wood were characterized for their optimal pH, temperature and stability to compare their effects on xylan extraction from pretreated sugarcane bagasse. On-site produced xylanase was prepared from cultures of the alkaliphilic Bacillus pumilus CBMAI 0008 strain. The optimal temperatures and pH levels for all commercial xylanases were 60 °C and 6, whereas for B. pumilus xylanase, they were 50 °C and 8, respectively. B. pumilus was active up to pH 10, but it had low thermal stability. Five sugarcane substrates were prepared to contain decreasing lignin content based on alkaline-sulfite pretreatment or acid-chlorite delignification. Xylanase doses (5–100 IU/g substrate) were applied to these substrates at pH 8.0, 50 °C and a 24-h reaction. Xylan extraction yields were strongly dependent on the lignin content of the substrates. Maximal xylan extraction yields were obtained in acid-chlorite delignified substrates, reaching values of 64% and 45% for Luminase and B. pumilus xylanases, respectively. Residual (non-extractable) xylans seemed occluded by and/or complexed with residual lignin of the substrates.
- Imprenta:
- Source:
- Título: EUBCE 2019 27th European Biomass Conference & Exhibition
- Volume/Número/Paginação/Ano: 2019
-
ABNT
SANTOS, Maiara Pedral dos et al. In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. 2019, Anais.. Lisboa: ETA, 2019. . Acesso em: 27 dez. 2025. -
APA
Santos, M. P. dos, Távilla, V., Reinoso, F. A. M., Ferraz, A. L., & Milagres , A. M. F. (2019). In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. In EUBCE 2019 27th European Biomass Conference & Exhibition. Lisboa: ETA. -
NLM
Santos MP dos, Távilla V, Reinoso FAM, Ferraz AL, Milagres AMF. In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. EUBCE 2019 27th European Biomass Conference & Exhibition. 2019 ;[citado 2025 dez. 27 ] -
Vancouver
Santos MP dos, Távilla V, Reinoso FAM, Ferraz AL, Milagres AMF. In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse. EUBCE 2019 27th European Biomass Conference & Exhibition. 2019 ;[citado 2025 dez. 27 ] - Sugarcane hybrids with original low lignin contents and high field productivity are useful to reach high glucose yields from bagasse
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- Comparative evaluation of acid and alkaline sulfite pretreatments for enzymatic saccharification of bagasses from three different sugarcane hybrids
- Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin
- Techno-economic impacts of varied compositional profiles of sugarcane experimental hybrids on a biorefinery producing sugar, ethanol and electricity
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