Artigo de periodico

The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures (2019)

• Authors:
  • Valadares, Fernanda de Lima
  • Gonçalves, Thiago Augusto
  • Damásio, André Ricardo de Lima
  • Milagres , Adriane Maria Ferreira
  • Squina, Fabio Marcio
  • Segato, Fernando
  • Ferraz, André Luis
• USP affiliated authors: MILAGRES, ADRIANE MARIA FERREIRA - EEL ; SEGATO, FERNANDO - EEL ; FERRAZ, ANDRÉ LUIS - EEL
• Unidade: EEL
• DOI: 10.1016/j.enzmictec.2019.109370
• Assunto: BIOTECNOLOGIA
• Keywords: Basidiomycetes; Biorefinery; CAZymes; Secretome; Xylanases; Wood decay
• Agências de fomento:
  • Financiamento CNPq
  • Financiamento CAPES
  • Financiamento FAPESP
• Language: Inglês
• Abstract: Secretome evaluations of lignocellulose-decay basidiomycetes can reveal new enzymes in selected fungal species that degrade specific substrates. Proteins discovered in such studies can support biorefinery development. Brown-rot (Gloeophyllum trabeum) and white-rot (Pleurotus ostreatus) fungi growing in sugarcane bagasse solidstate cultures produced 119 and 63 different extracellular proteins, respectively. Several of the identified enzymes are suitable for in vitro biomass conversion, including a range of cellulases (endoglucanases, cellobiohydrolases and β-glucosidases), hemicellulases (endoxylanases, α-arabinofuranosidases, α-glucuronidases and acetylxylan esterases) and carbohydrate-active auxiliary proteins, such as AA9 lytic polysaccharide monooxygenase, AA1 laccase and AA2 versatile peroxidase. Extracellular oxalate decarboxylase was also detected in both fungal species, exclusively in media containing sugarcane bagasse. Interestingly, intracellular AA6 quinone oxidoreductases were also exclusively produced under sugarcane bagasse induction in both fungi. These enzymes promote quinone redox cycling, which is used to produce Fenton’s reagents by lignocellulose-decay fungi. Hitherto undiscovered hypothetical proteins that are predicted in lignocellulose-decay fungi genomes appeared in high relative abundance in the cultures containing sugarcane bagasse, which suggests undisclosed, new biochemical mechanisms that are used by lignocellulose-decay fungi to degrade sugarcane biomass. In general, lignocellulose-decay fungi produce a number of canonical hydrolases, as well as some newly observed enzymes, that are suitable for in vitro biomass digestion in a biorefinery context.
• Imprenta:
  • Publisher: Elsevier
  • Publisher place: Seul
  • Date published: 2019
• Source:
  • Título: Enzyme and microbial technology
  • ISSN: 0141-0229
  • Volume/Número/Paginação/Ano: v.130, art.109370, pp.1-10, 2019

Informações sobre o DOI: 10.1016/j.enzmictec.2019.109370 (Fonte: oaDOI API)
• Este periódico é de assinatura
• Este artigo NÃO é de acesso aberto
  
• Cor do Acesso Aberto: closed

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How to cite

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• ABNT

  VALADARES, Fernanda de Lima et al. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures. Enzyme and microbial technology, v. 130, p. p1-10, 2019Tradução . . Disponível em: https://doi.org/10.1016/j.enzmictec.2019.109370. Acesso em: 27 dez. 2025.

• APA

  Valadares, F. de L., Gonçalves, T. A., Damásio, A. R. de L., Milagres , A. M. F., Squina, F. M., Segato, F., & Ferraz, A. L. (2019). The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures. Enzyme and microbial technology, 130, p1-10. doi:10.1016/j.enzmictec.2019.109370

• NLM

  Valadares F de L, Gonçalves TA, Damásio AR de L, Milagres AMF, Squina FM, Segato F, Ferraz AL. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures [Internet]. Enzyme and microbial technology. 2019 ;130 p1-10.[citado 2025 dez. 27 ] Available from: https://doi.org/10.1016/j.enzmictec.2019.109370

• Vancouver

  Valadares F de L, Gonçalves TA, Damásio AR de L, Milagres AMF, Squina FM, Segato F, Ferraz AL. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures [Internet]. Enzyme and microbial technology. 2019 ;130 p1-10.[citado 2025 dez. 27 ] Available from: https://doi.org/10.1016/j.enzmictec.2019.109370

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