Continuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport (2021)
- Authors:
- USP affiliated authors: CASTRO, HEIZIR FERREIRA DE - EEL ; PINTO, PEDRO AUGUSTO RAMOS - EEL ; CARVALHO, ANA KARINE FURTADO DE - EEL
- Unidade: EEL
- DOI: 10.1007/s10562-021-03826-y
- Assunto: BIOTECNOLOGIA
- Keywords: Enzyme immobilization; Continuous reaction; Magnetic nanoparticles
- Agências de fomento:
- Language: Inglês
- Abstract: This study deals with the use of commercial lipase stabilized onto magnetic particles of iron oxides (Fe3O4/γ-Fe2O3) and applied as a heterogeneous biocatalyst in the synthesis of ethyl esters (biodiesel) in a solvent-free medium on a continuous process. Magnetic particles were synthesized by alkaline co-precipitation, silanized, activated and subsequently used for immobilization of Burkholderia cepacia lipase. The results regarding the lipase immobilization showed an enzyme activity retention of about 53% and an increase in the KM value in about threefold (from 410 to 1262 mmol L−1) and a decrease in the Vmax value in sevenfold (From 12,390 to 1786 U g−1) when compared with the free enzyme. The immobilization process favored the thermal stability and increased the half-time of the enzyme about tenfold at 50 °C. The immobilized derivative was evaluated to aroma production and the activity of esterification was calculated as being 56.7 μmol L−1 g−1 min−1, which corresponds to a productivity value of 0.58 g ester L−1 h−1. The immobilized system was also used to mediate transesterification of kernel oil in a fixed bed reactor operating in a continuous flow with a reaction medium composed of oil and ethanol at a molar ratio of 1:12, 50 °C and space–time of 16 h. The operational stability of the immobilized lipase estimated at 47 days allowed to operate the reactor with high productivity of 38.7 ± 0.7 mg g−1 h−1. Also, the product properties of the ester content (> 96.5%) and kinematic viscosity value (5.32 ± 0.4 mm2 s−1 at 40 °C) meet the requirements of the ANP and ASTM (D6751) for biodiesel fuel.
- Imprenta:
- Source:
- Título: Catalysis letters
- ISSN: 1011-372X
- Volume/Número/Paginação/Ano: v.152, p.2434–2444, 2021
- Este periódico é de acesso aberto
- Este artigo NÃO é de acesso aberto
-
ABNT
BENTO, Heitor Buzetti Simões et al. Continuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport. Catalysis letters, v. 152, p. 2434–2444, 2021Tradução . . Disponível em: https://doi.org/10.1007/s10562-021-03826-y. Acesso em: 25 fev. 2026. -
APA
Bento, H. B. S., Reis, C. E. R., Pinto, P. A. R., Cortez, D. V., Boas, R. N. V., Costa-Silva, T. A., et al. (2021). Continuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport. Catalysis letters, 152, 2434–2444. doi:10.1007/s10562-021-03826-y -
NLM
Bento HBS, Reis CER, Pinto PAR, Cortez DV, Boas RNV, Costa-Silva TA, Carvalho AKF, Castro HF de. Continuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport [Internet]. Catalysis letters. 2021 ;152 2434–2444.[citado 2026 fev. 25 ] Available from: https://doi.org/10.1007/s10562-021-03826-y -
Vancouver
Bento HBS, Reis CER, Pinto PAR, Cortez DV, Boas RNV, Costa-Silva TA, Carvalho AKF, Castro HF de. Continuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport [Internet]. Catalysis letters. 2021 ;152 2434–2444.[citado 2026 fev. 25 ] Available from: https://doi.org/10.1007/s10562-021-03826-y - Penicillium citrinum whole-cells catalyst for the treatment of lipid-rich wastewater
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Informações sobre o DOI: 10.1007/s10562-021-03826-y (Fonte: oaDOI API)
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