Trabalho de evento-resumo

Evaluation effect of recombinant lytic polysaccharide monooxygenases (LPMOs) from family AA9 on ligninocellulosic materials (2023)

• Authors:
  • Gutierrez, Solange G. Barrios
  • Berto, Gabriela Leila
  • Ferraz, André Luis
  • Sanchez-Muñoz, Salvador
  • Segato, Fernando
• USP affiliated authors: FERRAZ, ANDRÉ LUIS - EEL ; SEGATO, FERNANDO - EEL ; GUTIERREZ, SOLANGE GRACE BARRIOS - EEL ; MUÑOZ, SALVADOR SANCHEZ - EEL ; BERTO, GABRIELA LEILA - EEL
• Unidade: EEL
• Subjects: MICROBIOLOGIA; LIGNINA
• Keywords: Protein expression; filamentous fungi; LPMO; Lignin
• Agências de fomento:
  • Financiamento CNPq
  • Financiamento FAPESP
  • Financiamento CAPES
• Language: Inglês
• Abstract: Lignin has long been considered a significant obstacle in biorefinery processes aiming to derive sustainable and viable bioproducts from lignocellulosic biomass. Its recalcitrance has spurred the search for more efficient and cost-effective treatments to modify and valorize this polymer, elevating its importance at an industrial level. In this context, the emergence of lytic polysaccharide monooxygenases (LPMOs), enzymes capable of breaking down glycosidic bonds through oxidation, has revolutionized our understanding of enzymatic degradation of polysaccharides, especially cellulose. LPMOs are predominantly found in filamentous fungi, such as ascomycetes and basidiomycetes, which are known for their ability to degrade plant biomass. Numerous genes encode different families of LPMOs within their genomes, with the AA9 family being the focus of this work. The objective of this study revolves around the recombinant production of LPMOs from these fungi, intending to apply them to modify lignin. To achieve our goals, the genes encoding the enzymes TtLPMO9C and TvAA9B from Thermothielavioides terrestris and Trametes versicolor, respectively, underwent analysis for amplification, cloning, and transformation into Aspergillus nidulans A773. The resulting transformants were employed to express the target proteins, which were subsequently purified using chromatography techniques. Following the purification, the two LPMOs underwent biochemical characterization, including examinations of their pH and optimal temperature range, as well as their thermostability, to gain deeper insights into the operational conditions of both LPMOs. Subsequently, their activity was analyzed through testing on 2,6-DMP and PASC, revealing their regioselectivity at C1, C4, and C1/C4 in cellulose. Furthermore, preliminary tests were performed to observe their impact on in natura pine biomass. These tests demonstrated the LPMOs’ capacity to modify the structure of plant biomass,as confirmed through Fluorescence Optical Microscopy. These findings underscore the significant potential of LPMOs in lignin modification.
• Imprenta:
  • Publisher: SBM
  • Publisher place: Foz do Iguaçú-PR
  • Date published: 2023
• Source:
  • Título: Anais do 32o Congresso Brasileiro de Microbiologia
  • Volume/Número/Paginação/Ano: n.863-1, 2023


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How to cite

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• ABNT

  GUTIERREZ, Solange G. Barrios et al. Evaluation effect of recombinant lytic polysaccharide monooxygenases (LPMOs) from family AA9 on ligninocellulosic materials. 2023, Anais.. Foz do Iguaçú-PR: SBM, 2023. Disponível em: https://sbmicrobiologia.org.br/32cbm-anais/resumos/R-0863-1.html. Acesso em: 27 dez. 2025.

• APA

  Gutierrez, S. G. B., Berto, G. L., Ferraz, A. L., Sanchez-Muñoz, S., & Segato, F. (2023). Evaluation effect of recombinant lytic polysaccharide monooxygenases (LPMOs) from family AA9 on ligninocellulosic materials. In Anais do 32o Congresso Brasileiro de Microbiologia. Foz do Iguaçú-PR: SBM. Recuperado de https://sbmicrobiologia.org.br/32cbm-anais/resumos/R-0863-1.html

• NLM

  Gutierrez SGB, Berto GL, Ferraz AL, Sanchez-Muñoz S, Segato F. Evaluation effect of recombinant lytic polysaccharide monooxygenases (LPMOs) from family AA9 on ligninocellulosic materials [Internet]. Anais do 32o Congresso Brasileiro de Microbiologia. 2023 ;(863-1):[citado 2025 dez. 27 ] Available from: https://sbmicrobiologia.org.br/32cbm-anais/resumos/R-0863-1.html

• Vancouver

  Gutierrez SGB, Berto GL, Ferraz AL, Sanchez-Muñoz S, Segato F. Evaluation effect of recombinant lytic polysaccharide monooxygenases (LPMOs) from family AA9 on ligninocellulosic materials [Internet]. Anais do 32o Congresso Brasileiro de Microbiologia. 2023 ;(863-1):[citado 2025 dez. 27 ] Available from: https://sbmicrobiologia.org.br/32cbm-anais/resumos/R-0863-1.html

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