Cellobiohydrolases from varied fungal sources are differentially affected by lignin-related compounds and insoluble lignin (2022)
- Authors:
- USP affiliated authors: SEGATO, FERNANDO - EEL ; FERRAZ, ANDRÉ LUIS - EEL ; OLIVA, BIANCA - EEL
- Unidade: EEL
- Subjects: BIOPROCESSOS; BIOTECNOLOGIA
- Keywords: Lignin-related compounds; inhibition; Cellobiohydrolases; Unproductive adsorption
- Agências de fomento:
- Language: Inglês
- Abstract: Lignocellulosic components found in plant biomass are an abundant, renewable and available source of energy and chemical compounds. The efficient conversion of this recalcitrant material in simple molecules requires the combined action of enzymes with hydrolytic and oxidative activities . Even after pre-treatment processes, the presence of residual lignin and lignin-related compounds (LRC) can hinder the action of cellulases during the cellulose depolymerization. The negative impact of this residual lignin in biomass saccharification can be assigned to several factors, as follow: (i) insoluble lignin deposited onto the fibers surface can block the access of cellulases to cellulose; (ii) cellulases can be unproductively adsorbed onto insoluble lignin (PINTO et al., 2021); and (iii) soluble LRC generated by lignin degradation in pre-treatment process can inactivate cellulases by precipitation and irreversible interaction (XIMENES et al., 2010). Due the interest to use high-solids loading in industrial processes, the effect of LRC on enzymatic saccharification of biomass has become an important issue. The negative effect of LRC on saccharification processes using commercial lignocellulolytic cocktails have been reported, but their interaction with isolated enzymes, such as cellobiohydrolases (CBHs), is still an open question.
- Source:
- Título: Proceedings of the XXIII SINAFERM & XIV SHEB & ENZITEC 2022
- ISSN: 2447-2816
- Volume/Número/Paginação/Ano: p, 1-2, 2022
-
ABNT
OLIVA, Bianca e FERRAZ, André Luis e SEGATO, Fernando. Cellobiohydrolases from varied fungal sources are differentially affected by lignin-related compounds and insoluble lignin. Proceedings of the XXIII SINAFERM & XIV SHEB & ENZITEC 2022. [S.l.]: Escola de Engenharia de Lorena, Universidade de São Paulo. . Acesso em: 13 fev. 2026. , 2022 -
APA
Oliva, B., Ferraz, A. L., & Segato, F. (2022). Cellobiohydrolases from varied fungal sources are differentially affected by lignin-related compounds and insoluble lignin. Proceedings of the XXIII SINAFERM & XIV SHEB & ENZITEC 2022. Escola de Engenharia de Lorena, Universidade de São Paulo. -
NLM
Oliva B, Ferraz AL, Segato F. Cellobiohydrolases from varied fungal sources are differentially affected by lignin-related compounds and insoluble lignin. Proceedings of the XXIII SINAFERM & XIV SHEB & ENZITEC 2022. 2022 ;[citado 2026 fev. 13 ] -
Vancouver
Oliva B, Ferraz AL, Segato F. Cellobiohydrolases from varied fungal sources are differentially affected by lignin-related compounds and insoluble lignin. Proceedings of the XXIII SINAFERM & XIV SHEB & ENZITEC 2022. 2022 ;[citado 2026 fev. 13 ] - Biochemical and inhibitor analysis of recombinant cellobiohydrolases from Phanerochaete chrysosporium
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