Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain (2020)
- Authors:
- Autor USP: SEGATO, FERNANDO - EEL
- Unidade: EEL
- DOI: 10.1016/j.btre.2020.e00457
- Assunto: ENZIMAS
- Keywords: Lote alimentado; Lote repedito; Xilanase; Otimização
- Agências de fomento:
- Language: Inglês
- Abstract: The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6 h−1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6 h−1. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.
- Imprenta:
- Source:
- Título: Biotechnology reports
- ISSN: 2215017X
- Volume/Número/Paginação/Ano: v., n. , p.e00457-, 2020
- Status:
- Artigo publicado em periódico de acesso aberto (Gold Open Access)
- Versão do Documento:
- Versão publicada (Published version)
- Acessar versão aberta:
-
ABNT
ABDELLA, Asmaa e SEGATO, Fernando e WILKINS, Mark R. Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain. Biotechnology reports, n. , p. e00457-, 2020Tradução . . Disponível em: https://doi.org/10.1016/j.btre.2020.e00457. Acesso em: 31 mar. 2026. -
APA
Abdella, A., Segato, F., & Wilkins, M. R. (2020). Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain. Biotechnology reports, ( ), e00457-. doi:10.1016/j.btre.2020.e00457 -
NLM
Abdella A, Segato F, Wilkins MR. Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain [Internet]. Biotechnology reports. 2020 ;( ): e00457-.[citado 2026 mar. 31 ] Available from: https://doi.org/10.1016/j.btre.2020.e00457 -
Vancouver
Abdella A, Segato F, Wilkins MR. Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain [Internet]. Biotechnology reports. 2020 ;( ): e00457-.[citado 2026 mar. 31 ] Available from: https://doi.org/10.1016/j.btre.2020.e00457 - Continuous xylanase production with Aspergillus nidulans under pyridoxine limitation using a trickle bed reactor
- Exploring lignin depolymerization by a bi-enzyme system containing aryl alcohol oxidase and lignin peroxidase in aqueous biocompatible ionic liquids
- Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor
- Feruloyl esterases: Biocatalysts to overcome biomass recalcitrance and for the production of bioactive compounds
- Optimization of nutrient medium components for production of a client endo-β-1,4-xylanase from Aspergillus fumigatus var. niveus using a recombinant Aspergillus nidulans strain
- Light activation of T. thermophilus LPMOs 9A and 9H, synergy with cellulases and application for cellulose nanofibers production
- Expressão gênica envolvida nos mecanismos de resistência a acriflavina, griseofulvina e terbinafina em fungos filamentosos
- Exoproteoma, clonagem, expressão e caracterização heteróloga de enzimas termofílicas do fungo Thielavia terrestris com aplicação biotecnológica
- System biology in lignocellulose and algae refineries
- Desenvolvimento de um cell factory a partir do fungo termofílico Thermothielavioides terrestris
Informações sobre a disponibilidade de versões do artigo em acesso aberto coletadas automaticamente via oaDOI API (Unpaywall).
Por se tratar de integração com serviço externo, podem existir diferentes versões do trabalho (como preprints ou postprints), que podem diferir da versão publicada.
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
