Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain (2020)
- Authors:
- Autor USP: SEGATO, FERNANDO - EEL
- Unidade: EEL
- DOI: 10.1016/j.btre.2020.e00457
- Assunto: ENZIMAS
- Keywords: Lote alimentado; Lote repedito; Xilanase; Otimização
- Agências de fomento:
- Language: Inglês
- Abstract: The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (kLa) for efficient xylanase production was found to be 38.6 h−1. Fed batch mode and repeated batch fermentation was also performed with kLa was 38.6 h−1. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.
- Imprenta:
- Source:
- Título: Biotechnology reports
- ISSN: 2215017X
- Volume/Número/Paginação/Ano: v., n. , p.e00457-, 2020
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by-nc-nd
-
ABNT
ABDELLA, Asmaa e SEGATO, Fernando e WILKINS, Mark R. Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain. Biotechnology reports, n. , p. e00457-, 2020Tradução . . Disponível em: https://doi.org/10.1016/j.btre.2020.e00457. Acesso em: 26 dez. 2025. -
APA
Abdella, A., Segato, F., & Wilkins, M. R. (2020). Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain. Biotechnology reports, ( ), e00457-. doi:10.1016/j.btre.2020.e00457 -
NLM
Abdella A, Segato F, Wilkins MR. Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain [Internet]. Biotechnology reports. 2020 ;( ): e00457-.[citado 2025 dez. 26 ] Available from: https://doi.org/10.1016/j.btre.2020.e00457 -
Vancouver
Abdella A, Segato F, Wilkins MR. Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain [Internet]. Biotechnology reports. 2020 ;( ): e00457-.[citado 2025 dez. 26 ] Available from: https://doi.org/10.1016/j.btre.2020.e00457 - Continuous xylanase production with Aspergillus nidulans under pyridoxine limitation using a trickle bed reactor
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Informações sobre o DOI: 10.1016/j.btre.2020.e00457 (Fonte: oaDOI API)
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