Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor (2021)
- Authors:
- Autor USP: SEGATO, FERNANDO - EEL
- Unidade: EEL
- DOI: 10.1016/j.biortech.2021.124700
- Assunto: BIOTECNOLOGIA
- Keywords: Lignin-degrading enzymes; Fed-batch fermentation; Fungal enzyme production; Heterologous protein expression; Process optimization
- Agências de fomento:
- Language: Inglês
- Abstract: Enzymatic lignin depolymerization is considered a favorable approach to utilize lignin due to the higher selectivity and less energy requirement when compared to thermochemical lignin valorization. Lignin peroxidase (LiP) is one of the key enzymes involved in lignin degradation and possesses high redox potential to oxidize non-phenolic structures and phenolic compounds in lignin. However, the production of LiP is mainly from white-rot fungi at small scales. It is critical to discover new LiP from other microorganisms and produce LiP at large scales. This study aims to produce a novel LiP originally from Thermothelomyces thermophiles using a recombinant Aspergillus nidulans strain. The LiP production medium was optimized, and different fed-batch strategies for LiP production were investigated to improve LiP activity, yield, and productivity. Results demonstrated that LiP production was enhanced by using multi-pulse fed-batch fermentation. A maximum LiP activity of 1,645 mU/L with a protein concentration of 0.26 g/L was achieved.
- Imprenta:
- Publisher: Elsevier
- Publisher place: Honolulu-HW
- Date published: 2021
- Source:
- Título: Bioresource technology
- ISSN: 0960-8524
- Volume/Número/Paginação/Ano: v.325, p.1-10, 2021
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
LIU, Enshi e SEGATO, Fernando e WILKINS, Mark R. Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor. Bioresource technology, v. 325, p. 1-10, 2021Tradução . . Disponível em: https://doi.org/10.1016/j.biortech.2021.124700. Acesso em: 26 dez. 2025. -
APA
Liu, E., Segato, F., & Wilkins, M. R. (2021). Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor. Bioresource technology, 325, 1-10. doi:10.1016/j.biortech.2021.124700 -
NLM
Liu E, Segato F, Wilkins MR. Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor [Internet]. Bioresource technology. 2021 ;325 1-10.[citado 2025 dez. 26 ] Available from: https://doi.org/10.1016/j.biortech.2021.124700 -
Vancouver
Liu E, Segato F, Wilkins MR. Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor [Internet]. Bioresource technology. 2021 ;325 1-10.[citado 2025 dez. 26 ] Available from: https://doi.org/10.1016/j.biortech.2021.124700 - Continuous xylanase production with Aspergillus nidulans under pyridoxine limitation using a trickle bed reactor
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Informações sobre o DOI: 10.1016/j.biortech.2021.124700 (Fonte: oaDOI API)
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