Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases (2018)
- Authors:
- USP affiliated authors: CASTRO, HEIZIR FERREIRA DE - EEL ; FREITAS, LARISSA DE - EEL ; AGUIAR, LEANDRO GONÇALVES DE - EEL ; SILVA, MATEUS VINICIUS CASAGRANDE DA - EEL
- Unidade: EEL
- Sigla do Departamento: LOQ
- DOI: 10.1007/s11274-018-2553-1
- Subjects: MATERIAIS MAGNÉTICOS; POLÍMEROS SINTÉTICOS
- Keywords: Biocatálise; Pinça Magnética; Immobilized enzymes; Biomethanol; Magnetic Materials; Polymer Synthesis; Magnetic tweezers; Biocatalysis
- Agências de fomento:
- Language: Inglês
- Abstract: The parameters that effect the synthesis of poly(styrene-co-divinylbenzene) magnetized with magnetite (STY-DVB-M) by polymerization emulsion were assessed in order to obtain magnetic beads to be used as matrix for lipase immobilization. The combined effect of polyvinyl alcohol (PVA) concentration and agitation was studied using response surface methodology. A 22 full-factorial design was employed for experimental design and analysis of the results. The optimum PVA concentration and agitation were found to be 1 wt% and 400 rpm, respectively. These conditions allow attaining the best particle size distribution of the synthesized particles (80% between 80 and 24 mesh). The performance of the magnetic beads was tested as a matrix for immobilizing two microbial lipases (Lipases from Burkholderia cepacia-BCL and Pseudomonas fluorescens-AKL) by physical adsorption and high immobilization yields (> 70%) and hydrolytic activities (≅ 1850 U g−1) were attained. The properties of free and immobilized lipases were searched and compared. Similar performance regarding the analyzed parameters (biochemical properties, kinetic constants and thermal stability) were obtained. Moreover, both immobilized lipases were found to be able to catalyze the transesterification of coconut oil with ethanol to produce fatty acid ethyl esters (FAEE). Further study showed that the B. cepacia immobilized lipase could be used seven times without significant decrease of activity, revealing half-life time of 970h.
- Imprenta:
- Source:
- Título: World journal of microbiology & biotechnology
- ISSN: 0959-3993
- Volume/Número/Paginação/Ano: v. 34, p.158-169, 2018
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
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ABNT
SILVA, Mateus Vinicius Casagrande da et al. Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases. World journal of microbiology & biotechnology, v. 34, p. 158-169, 2018Tradução . . Disponível em: https://doi.org/10.1007/s11274-018-2553-1. Acesso em: 28 dez. 2025. -
APA
Silva, M. V. C. da, Aguiar, L. G. de, Castro, H. F. de, & Freitas, L. de. (2018). Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases. World journal of microbiology & biotechnology, 34, 158-169. doi:10.1007/s11274-018-2553-1 -
NLM
Silva MVC da, Aguiar LG de, Castro HF de, Freitas L de. Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases [Internet]. World journal of microbiology & biotechnology. 2018 ; 34 158-169.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1007/s11274-018-2553-1 -
Vancouver
Silva MVC da, Aguiar LG de, Castro HF de, Freitas L de. Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases [Internet]. World journal of microbiology & biotechnology. 2018 ; 34 158-169.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1007/s11274-018-2553-1 - Kinetic study of isopropyl palmitate synthesis catalyzed by lipases immobilized on a magnetic copolymer support
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Informações sobre o DOI: 10.1007/s11274-018-2553-1 (Fonte: oaDOI API)
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