Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling (2020)
- Authors:
- USP affiliated authors: CASTRO, HEIZIR FERREIRA DE - EEL ; AGUIAR, LEANDRO GONÇALVES DE - EEL ; FREITAS, LARISSA DE - EEL ; SILVA, MATEUS VINICIUS CASAGRANDE DA - EEL ; RANGEL, AMANDA BAHIA DE SOUZA - EEL
- Unidade: EEL
- DOI: 10.1021/acs.iecr.0c03504
- Subjects: BIOTECNOLOGIA; TRANSPORTE DE MASSA; COMPOSTOS ORGÂNICOS; PEPTÍDEOS
- Keywords: Porosity; Biotechnology; Mass transfer; Organic compounds; Peptides and proteins
- Agências de fomento:
- Language: Inglês
- Abstract: The continuous synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) particles was performed in a solvent-free fluidized bed reactor (FBR). The effects of space time (6, 12, and 18 h) and bed porosity (0.866, 0.892, and 0.916) were investigated to determine the best operating conditions. Experimental results showed that high ester productivity (0.73 mmol g–1 h–1) and a satisfactory esterification yield (48.24%) were achieved at a space time of 12 h and a bed porosity of 0.892. It was also observed that the higher the bed porosity, the higher the mass transfer coefficient and, consequently, the reactor productivity. This finding can be explained by the high interstitial velocity of the fluidized bed bioreactor, which increases the mixing degree and improves mass transfer. A mathematical model accounting for internal and external particle transfer effects was proposed based on ping-pong bi–bi enzyme kinetics. The FBR was described in a simplified manner, considering an agitated tank reactor, a strategy that provided good correlation (R2 = 0.991) between experimental and model results. Immobilized lipase showed high operational stability (half-life of 1716 h) qualifying as a promising biocatalyst for industrial-scale applications.
- Imprenta:
- Publisher: ACS Publications
- Publisher place: Washington-DC
- Date published: 2020
- Source:
- Título: Industrial & engineering chemistry research
- ISSN: 0888-5885
- Volume/Número/Paginação/Ano: v. 59, n. 44, p.19522-19530, 2020
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
SILVA, Mateus Vinicius Casagrande da et al. Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling. Industrial & engineering chemistry research, v. 59, n. 44, p. 19522-19530, 2020Tradução . . Disponível em: https://doi.org/10.1021/acs.iecr.0c03504. Acesso em: 04 ago. 2025. -
APA
Silva, M. V. C. da, Rangel, A. B. de S., Aguiar, L. G. de, Castro, H. F. de, & Freitas, L. de. (2020). Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling. Industrial & engineering chemistry research, 59( 44), 19522-19530. doi:10.1021/acs.iecr.0c03504 -
NLM
Silva MVC da, Rangel AB de S, Aguiar LG de, Castro HF de, Freitas L de. Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling [Internet]. Industrial & engineering chemistry research. 2020 ; 59( 44): 19522-19530.[citado 2025 ago. 04 ] Available from: https://doi.org/10.1021/acs.iecr.0c03504 -
Vancouver
Silva MVC da, Rangel AB de S, Aguiar LG de, Castro HF de, Freitas L de. Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling [Internet]. Industrial & engineering chemistry research. 2020 ; 59( 44): 19522-19530.[citado 2025 ago. 04 ] Available from: https://doi.org/10.1021/acs.iecr.0c03504 - Estudo cinético e termodinâmico da síntese do oleato de 2-etilhexila catalisada por lipase B de Candida antarctica imobilizada em copolímeros magnéticos
- Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate
- Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow
- Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases
- Síntese do oleato de 2-etilhexila catalisada pela lipase imobilizada em suporte magnético conduzida em biorreator de leito fluidizado
- Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support
- Estudo cinético e termodinâmico da síntese do oleato de 2-etilhexila catalisada por lipase imobilizada em partículas magnetizadas de poli(estireno-co-dimetacrilato de etilenoglicol)
- Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization
- Kinetic study of isopropyl palmitate synthesis catalyzed by lipases immobilized on a magnetic copolymer support
- Kinetic study of isopropyl palmitate synthesis catalyzed by lipases immobilized on a magnetic copolymer support
Informações sobre o DOI: 10.1021/acs.iecr.0c03504 (Fonte: oaDOI API)
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