Artigo de periodico

Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow (2020)

• Authors:
  • Silva, Mateus Vinicius Casagrande da
  • Rangel, Amanda Bahia de Souza
  • Castro, Heizir Ferreira de
  • Aguiar, Leandro Gonçalves de
  • Oliveira, Pedro Carlos de
  • Freitas, Larissa de
• USP affiliated authors: CASTRO, HEIZIR FERREIRA DE - EEL ; AGUIAR, LEANDRO GONÇALVES DE - EEL ; OLIVEIRA, PEDRO CARLOS DE - EEL ; FREITAS, LARISSA DE - EEL ; SILVA, MATEUS VINICIUS CASAGRANDE DA - EEL ; RANGEL, AMANDA BAHIA DE SOUZA - EEL
• Unidade: EEL
• DOI: 10.1007/s00449-019-02257-9
• Subjects: CINÉTICA; LIPASE; ESTERIFICAÇÃO
• Keywords: Kinetic modeling; Esterifcation; Immobilized lipase; Magnetic polymer; Packed-bed reactor
• Agências de fomento:
  • Financiamento CAPES
  • Financiamento FAPESP
• Language: Inglês
• Abstract: This study investigated the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) particles in a continuous packed-bed bioreactor. Runs were carried out in a solvent-free system at 50 °C. The performance of the reactor was evaluated for substrates composed by oleic acid and 2-ethylhexanol at five molar ratios (1:4–4:1), determining its operation limits in terms of substrate flow rate. The system performance was quantified for three different flow rates corresponding to space-time between 3 and 12 h. For each condition, the influence of the space-time in the ester formation, esterification yield and productivity was determined. The molar ratio of acid-to-alcohol interfered, in a remarkable way, in the formation of 2-ethylhexyl oleate and the best performance was attained for substrate at equimolar ratio running at 12 h space-time. Under this condition, average 2-ethylhexyl oleate concentration was 471.65 ± 2.98 g L−1 which corresponded to ester productivity of 23.16 ± 0.49 mmol g−1 L−1 h−1. This strategy also gave high biocatalyst operational stability, revealing a half-life time of 2063 h. A model based on the ping-pong Bi–Bi mechanism was developed to describe the kinetics of the esterification reaction and validated using experimental data. The goodness of fit of the model was satisfactory (R2 = 0.9310–0.9952).
• Imprenta:
  • Publisher: Springer
  • Publisher place: Munique
  • Date published: 2020
• Source:
  • Título: Bioprocess and biosystems engineering (internet)
  • ISSN: 1615-7605
  • Volume/Número/Paginação/Ano: v. 43, p.615-623, 2020

Informações sobre o DOI: 10.1007/s00449-019-02257-9 (Fonte: oaDOI API)
• Este periódico é de assinatura
• Este artigo NÃO é de acesso aberto
  
• Cor do Acesso Aberto: closed

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• ABNT

  SILVA, Mateus Vinicius Casagrande da et al. Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow. Bioprocess and biosystems engineering (internet), v. 43, p. 615-623, 2020Tradução . . Disponível em: https://doi.org/10.1007/s00449-019-02257-9. Acesso em: 28 dez. 2025.

• APA

  Silva, M. V. C. da, Rangel, A. B. de S., Castro, H. F. de, Aguiar, L. G. de, Oliveira, P. C. de, & Freitas, L. de. (2020). Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow. Bioprocess and biosystems engineering (internet), 43, 615-623. doi:10.1007/s00449-019-02257-9

• NLM

  Silva MVC da, Rangel AB de S, Castro HF de, Aguiar LG de, Oliveira PC de, Freitas L de. Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow [Internet]. Bioprocess and biosystems engineering (internet). 2020 ; 43 615-623.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1007/s00449-019-02257-9

• Vancouver

  Silva MVC da, Rangel AB de S, Castro HF de, Aguiar LG de, Oliveira PC de, Freitas L de. Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow [Internet]. Bioprocess and biosystems engineering (internet). 2020 ; 43 615-623.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1007/s00449-019-02257-9

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