Artigo de periodico

Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support (2021)

• Authors:
  • Silva, Mateus Vinicius Casagrande da
  • Rangel, Amanda Bahia de Souza
  • Dutra, Fellipe P.
  • Castro, Heizir Ferreira de
  • Freitas, Larissa de
• USP affiliated authors: CASTRO, HEIZIR FERREIRA DE - EEL ; FREITAS, LARISSA DE - EEL ; DUTRA, FELLIPE POTZIK - EEL ; SILVA, MATEUS VINICIUS CASAGRANDE DA - EEL
• Unidade: EEL
• DOI: 10.1007/s10562-020-03391-w
• Subjects: ESTERIFICAÇÃO; LIPASE
• Keywords: Kinetic/thermodynamic studies; Esterifcation; Operational stability; Immobilized lipase; Magnetic hybrid polymer
• Agências de fomento:
  • Financiamento CAPES
  • Financiamento FAPESP
• Language: Inglês
• Abstract: This study investigated the kinetic and thermodynamic parameters of the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase (CALB) immobilized on magnetic poly(styrene-co-divinylbenzene) (STY-DVB-M). The influence of acid/alcohol molar ratio and temperature on oleic acid conversion, ester concentration, and productivity was also assessed. Excess of one of the reagents resulted in high conversions, but the highest productivities were obtained at the stoichiometric ratio. Thus, a 1:1 molar ratio was considered optimal. Thermodynamic parameters calculated from the van’t Hoff equation (ΔS =  + 850.64 J mol−1 K−1, ΔH =  + 273.78 kJ mol−1) revealed that the reaction is endothermic with a positive entropy change. Temperatures below 45 °C negatively affected reaction kinetics, explained by the positive ΔG values. CALB-STY-DVB-M showed a hydrolytic activity of 522.97 ± 19.14 U g−1 and was about 1.7 times more thermally stable at 60 °C than the free enzyme. In an operational stability test, conducted for 7 consecutive cycles of 24 h, the biocatalyst had a half-life of 739 h. CALB-STY-DVB-M showed satisfactory performance in the synthesis of 2-ethylhexyl oleate, providing oleic acid conversions close to 100%, ester concentration of 450 g L−1, and productivity of 0.5 mmol g−1 h−1. The biocatalyst holds promise for industrial applications.
• Imprenta:
  • Publisher: Springer
  • Date published: 2021
• Source:
  • Título: Catalysis letters
  • ISSN: 1011-372X
  • Volume/Número/Paginação/Ano: v.151, p.1239-1247, 2021

Informações sobre o DOI: 10.1007/s10562-020-03391-w (Fonte: oaDOI API)
• Este periódico é de assinatura
• Este artigo NÃO é de acesso aberto
  
• Cor do Acesso Aberto: closed

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• ABNT

  SILVA, Mateus Vinicius Casagrande da et al. Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support. Catalysis letters, v. 151, p. 1239-1247, 2021Tradução . . Disponível em: https://doi.org/10.1007/s10562-020-03391-w. Acesso em: 28 dez. 2025.

• APA

  Silva, M. V. C. da, Rangel, A. B. de S., Dutra, F. P., Castro, H. F. de, & Freitas, L. de. (2021). Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support. Catalysis letters, 151, 1239-1247. doi:10.1007/s10562-020-03391-w

• NLM

  Silva MVC da, Rangel AB de S, Dutra FP, Castro HF de, Freitas L de. Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support [Internet]. Catalysis letters. 2021 ;151 1239-1247.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1007/s10562-020-03391-w

• Vancouver

  Silva MVC da, Rangel AB de S, Dutra FP, Castro HF de, Freitas L de. Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support [Internet]. Catalysis letters. 2021 ;151 1239-1247.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1007/s10562-020-03391-w

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