Production of cellulases by Aureobasidium pullulans</i> LB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars (2021)
- Authors:
- USP affiliated authors: SEGATO, FERNANDO - EEL ; SILVA, SILVIO SILVERIO DA - EEL ; KUMAR, ANUJ - EEL ; KADOGUCHI, ELEN AYUMI - EEL ; VIEIRA, MATHEUS MAITAN - EEL
- Unidade: EEL
- DOI: 10.1080/10826068.2020.1799393
- Subjects: ENZIMAS HIDROLÍTICAS; BIOTECNOLOGIA
- Keywords: Aureobasidium pullulans; cellulase; cellulosic sugars; enzymatic hydrolysis; submerged fermentation
- Agências de fomento:
- Language: Inglês
- Abstract: Aureobasidium pullulans LB83 was evaluated for cellulase production under submerged fermenta-tion conditions. Different process variables such as carbon sources (corn cob, sugarcane bagasse,and sugarcane straw), synthetic (urea, ammonium sulfate, and peptone), and non-synthetic (soy-bean meal, rice, and corn meal) nitrogen sources and inoculum size were evaluated by one par-ameter at-a-time strategy. Aureobasidium pullulans LB83 showed maximum cellulase activity(FPase, 2.27 U/mL; CMCase, 7.42 U/mL) on sugarcane bagasse. Among the nitrogen sources, soy-bean meal as a non-synthetic nitrogen sources showed a maximum cellulase activity (FPase2.45 U/mL; CMCase, 6.86 U/mL) after 60 hr. The inoculum size of 1.6 106 CFU/mL had the max-imum FPase and CMCase activities of 3.14 and 8.74 U/mL, respectively. For the enzymatic hydroly-sis, both the commercial cellulase (10 FPU/g of Cellic CTec 2 (#A) and 10 FPU/g of crude enzymeextract (CEE) (#B), and varying ratio of CTec 2 and CEE in combination #C (5 FPU/g of CTec 2 þ 5FPU/g CEE), combination #D (2.5 FPU/g of CTec 2 þ 7.5 FPU/g CEE), and combination #E (7.5 FPU/g of CTec 2 þ 2.5 FPU/g CEE) were assessed for enzymatic hydrolysis of delignified sugarcanebagasse. Enzyme combination #C showed maximum hydrolysis yield of 92.40%. The study showsthe hydrolytic potential of cellulolytic enzymes from A. pullulans LB83 for lignocellulosic sugarsproduction from delignified sugarcane bagasse.
- Imprenta:
- Publisher: Taylor & Francis
- Publisher place: Londres
- Date published: 2021
- Source:
- Título: Preparative biochemistry and biotechnology
- ISSN: 1532-2297
- Volume/Número/Paginação/Ano: v. 51, n. 2, p. 153-163, 2021
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
VIEIRA, Matheus Maitan et al. Production of cellulases by Aureobasidium pullulans</i> LB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars. Preparative biochemistry and biotechnology, v. 51, n. 2, p. 153-163, 2021Tradução . . Disponível em: https://doi.org/10.1080/10826068.2020.1799393. Acesso em: 29 dez. 2025. -
APA
Vieira, M. M., Kadaguchi, E., Segato, F., Silva, S. S. da, & Chandel, A. K. (2021). Production of cellulases by Aureobasidium pullulans</i> LB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars. Preparative biochemistry and biotechnology, 51( 2), 153-163. doi:10.1080/10826068.2020.1799393 -
NLM
Vieira MM, Kadaguchi E, Segato F, Silva SS da, Chandel AK. Production of cellulases by Aureobasidium pullulans</i> LB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars [Internet]. Preparative biochemistry and biotechnology. 2021 ; 51( 2): 153-163.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1080/10826068.2020.1799393 -
Vancouver
Vieira MM, Kadaguchi E, Segato F, Silva SS da, Chandel AK. Production of cellulases by Aureobasidium pullulans</i> LB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars [Internet]. Preparative biochemistry and biotechnology. 2021 ; 51( 2): 153-163.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1080/10826068.2020.1799393 - Production of cellulases by Aureobasidium pullulans</i> LB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars
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Informações sobre o DOI: 10.1080/10826068.2020.1799393 (Fonte: oaDOI API)
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