Analysis of Aureobasidium pullulans</i> LB83 secretome reveals distinct carbohydrate active enzymes for biomass saccharification (2023)
- Authors:
- USP affiliated authors: SILVA, SILVIO SILVERIO DA - EEL ; SEGATO, FERNANDO - EEL ; KUMAR, ANUJ - EEL
- Unidade: EEL
- DOI: 10.1080/10826068.2023.2279109
- Assunto: BIOTECNOLOGIA
- Keywords: Aureobasidium pullulans; cellulase; CAZymes; submerged fermentation; secretome analysis
- Agências de fomento:
- Language: Inglês
- Abstract: Aureobasidium pullulans LB83 is a versatile biocatalyst that produces a plethora of bioactive products thriving on a variety of feedstocks under the varying culture conditions. In our last study using this microorganism, we found cellulase activity (FPase, 2.27 U/ml; CMCase, 7.42 U/ml) and other plant cell wall degrading enzyme activities grown on sugarcane bagasse and soybean meal as carbon source and nitrogen, respectively. In the present study, we provide insights on the secretome analysis of this enzymatic cocktail. The secretome analysis of A. pullulans LB83 by Liquid Chromatography coupled to Mass Spectroscopy (LC-MS/MS) revealed 38 classes of Carbohydrate Active enZymes (CAZymes) of a total of 464 identified proteins. These CAZymes consisted of 21 glycoside hydrolases (55.26%), 12 glycoside hydrolases harboring carbohydrate-binding module (31.58%), 4 carbohydrate esterases (10.53%) and one glycosyl transferase (2.63%). To the best of our knowledge, this is the first report on the secretome analysis of A. pullulans LB83.
- Imprenta:
- Publisher: Taylor & Francis
- Publisher place: Londres
- Date published: 2023
- Source:
- Título: Preparative biochemistry & biotechnology
- ISSN: 1082-6068
- Volume/Número/Paginação/Ano: v.53, n.10, p.1-7, 2023
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
VIEIRA, Matheus Maitan et al. Analysis of Aureobasidium pullulans</i> LB83 secretome reveals distinct carbohydrate active enzymes for biomass saccharification. Preparative biochemistry & biotechnology, v. 53, n. 10, p. 1-7, 2023Tradução . . Disponível em: https://doi.org/10.1080/10826068.2023.2279109. Acesso em: 29 dez. 2025. -
APA
Vieira, M. M., Valadares, F. de L., Mendoza, J. A. V., Silva, S. S. da, Segato, F., & Chandel, A. K. (2023). Analysis of Aureobasidium pullulans</i> LB83 secretome reveals distinct carbohydrate active enzymes for biomass saccharification. Preparative biochemistry & biotechnology, 53( 10), 1-7. doi:10.1080/10826068.2023.2279109 -
NLM
Vieira MM, Valadares F de L, Mendoza JAV, Silva SS da, Segato F, Chandel AK. Analysis of Aureobasidium pullulans</i> LB83 secretome reveals distinct carbohydrate active enzymes for biomass saccharification [Internet]. Preparative biochemistry & biotechnology. 2023 ;53( 10): 1-7.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1080/10826068.2023.2279109 -
Vancouver
Vieira MM, Valadares F de L, Mendoza JAV, Silva SS da, Segato F, Chandel AK. Analysis of Aureobasidium pullulans</i> LB83 secretome reveals distinct carbohydrate active enzymes for biomass saccharification [Internet]. Preparative biochemistry & biotechnology. 2023 ;53( 10): 1-7.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1080/10826068.2023.2279109 - Analysis of Aureobasidium pullulans</i> LB83 secretome reveals distinct carbohydrate active enzymes for biomass saccharification
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Informações sobre o DOI: 10.1080/10826068.2023.2279109 (Fonte: oaDOI API)
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