Optimization rhodotorula glutinis cultivation conditions for sigle- cell-oil production from glycerol (2019)
- Authors:
- USP affiliated authors: SILVA, JOÃO PAULO ALVES - EEL ; PEREIRA, VALDIMIR DOS SANTOS - EEL
- Unidade: EEL
- Assunto: BIOTECNOLOGIA
- Language: Inglês
- Abstract: The search of technologies that aim the use of industrial waste and by-products from established processes is a strategic point for the development of more efficient and sustainable industries. In this context, the alternative search for the use of glycerol, the main biodiesel production by-product, have an important role in the design of lipid-based biorefineries. Glycerol can be used in biotechnological processes as a substrate to obtain various products, among them microbial oils. Thus, the present study aimed to evaluate the influence of culture conditions on lipids production by the yeast Rhodotorula glutinis NRRLY-12905. The experiments were carried out according to surface methodology, using facecentered 24 full factorial design. In this experimental design were evaluated the effects of substrate concentration (40 to 200 g/L), carbon/nitrogen ratio (20:1 to 100:1), pH (5 to 7) and inoculum concentration (1 to 5 g/L) on the production of lipids. The assays were conducted in 250 mL Erlenmeyer flasks, containing 50 mL of culture medium, incubated in an orbital shaker at 200 rpm, at 30 ° C for 240 h. The culture medium was composed by MgSO4.7H2O (1 g/L), KH2PO4 (20 g/L), and glycerol, yeast extract and (NH4)2HPO4 (according to the experimental design). Within the evaluated region, the substrate concentration, pH and carbon/nitrogen ratio (C/N) showed statistically significant effects on lipid production. Among these variables, the substrate concentration and pH presented a quadratic behavior, with a maximum lipid accumulation of approximately 140 g/L and pH 6.5, respectively. The effect of the C/N ratio showed a positive behavior in the accumulation of lipids, that is, the increase in the C/N ratio led to an increase in the accumulation of lipids by yeast. Statistical analysis indicated that maximum lipid production by yeast R. glutinis was predicted for cultures with initial glycerolconcentration of 140 g/L, C/N ratio of 100 and pH of 6.5. Cultures performed under these conditions presented a cell concentration of about 30 ± 1 g/L after 200 h of cultivation, reaching accumulation of lipids of approximately 15 ± 3 g/L in the same period. In general, the study indicated that lipid production by yeast R. glutinis was favored in culture under high concentrations of glycerol and restrictions of nitrogen sources. The fatty acid profile showed that microbial oil is constituted mainly of oleic acid (31 to 36%), stearic acid (18 to 25%), linoleic acid (18 to 20%) and palmitic acid (20 to 23%). The fatty acids of eighteen and sixteen carbons correspond to 95% of the fatty acid profile of microbial oil. These results contribute to establishing operational conditions that maximize singlecell oil production from glycerol by Rhodotorula glutinis, i.e. an alternative source as renewable raw material for lipid-based biorefineries.
- Imprenta:
- Publisher: Universidade Brasil
- Publisher place: São José dos Campos-SP
- Date published: 2019
- Source:
- Título: Caderno de Resumos - III International Symposium on Fungal Stress
- Volume/Número/Paginação/Ano: p.94-95, 2019
-
ABNT
FERREIRA, Douglas dos Santos e PEREIRA, Valdimir dos Santos e SILVA, João Paulo Alves. Optimization rhodotorula glutinis cultivation conditions for sigle- cell-oil production from glycerol. 2019, Anais.. São José dos Campos-SP: Universidade Brasil, 2019. p. 94-95. Disponível em: http://www.even3.com.br/anais/ISFUS. Acesso em: 27 dez. 2025. -
APA
Ferreira, D. dos S., Pereira, V. dos S., & Silva, J. P. A. (2019). Optimization rhodotorula glutinis cultivation conditions for sigle- cell-oil production from glycerol. In Caderno de Resumos - III International Symposium on Fungal Stress (p. 94-95). São José dos Campos-SP: Universidade Brasil. Recuperado de http://www.even3.com.br/anais/ISFUS -
NLM
Ferreira D dos S, Pereira V dos S, Silva JPA. Optimization rhodotorula glutinis cultivation conditions for sigle- cell-oil production from glycerol [Internet]. Caderno de Resumos - III International Symposium on Fungal Stress. 2019 ;94-95.[citado 2025 dez. 27 ] Available from: http://www.even3.com.br/anais/ISFUS -
Vancouver
Ferreira D dos S, Pereira V dos S, Silva JPA. Optimization rhodotorula glutinis cultivation conditions for sigle- cell-oil production from glycerol [Internet]. Caderno de Resumos - III International Symposium on Fungal Stress. 2019 ;94-95.[citado 2025 dez. 27 ] Available from: http://www.even3.com.br/anais/ISFUS - Influence of aeration and substrate concentration on carotenoids and lipid production by Rhodotorula glutinis from glycerol
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