Laccase Activation in Deep Eutectic Solvents (2019)
- Authors:
- Autor USP: SILVA, JOÃO PAULO ALVES - EEL
- Unidade: EEL
- DOI: 10.1021/acssuschemeng.9b02179
- Assunto: ENZIMAS
- Keywords: deep eutectic solvents; enzyme activity; green solvents; laccase; molecular docking
- Language: Inglês
- Abstract: The research on alternative solvents and cosolvents is relevant when envisioning the improvement of biocatalytic reactions. Among these solvents and cosolvents, deep eutectic solvents (DES) may be considered as customizable new reaction media for biocatalysis. Accordingly, in this work, 16 DES aqueous solutions, as well as the individual DES components at the same conditions, have been investigated in laccase-catalyzed reactions. Cholinium- and betaine-based DES formed with polyols at different molar ratios and concentrations were evaluated. The results reported show that in the presence of most DES the laccase activity is preserved and, with a particular DES, enhanced up to 200%. Molecular docking studies demonstrated that while most DES components establish hydrogen bonds with the enzyme amino acids, those that establish stronger interactions with the enzyme (expressed by absolute values of docking affinity energies) lead to an enhanced laccase activity. Finally, the laccase stability was evaluated in additional tests under extreme storage temperatures (60 °C and −80 °C). Although no significant protection to high temperatures was afforded by DES, an enhanced laccase activity when stored at low temperatures was found, at least up to 20 days. Combining experimental results and molecular docking, this work shows that DES can be designed as cosolvents to improve biocatalytic reactions.
- Imprenta:
- Publisher place: Whashington-DC
- Date published: 2019
- Source:
- Título do periódico: ACS Sustainable Chemistry & Engineering
- ISSN: 21680485
- Volume/Número/Paginação/Ano: v.7, p.11806-11814, 2019
- Este periódico é de assinatura
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: green
- Licença: other-oa
-
ABNT
TOLEDO, Mariah L. et al. Laccase Activation in Deep Eutectic Solvents. ACS Sustainable Chemistry & Engineering, v. 7, p. 11806-11814, 2019Tradução . . Disponível em: https://doi.org/10.1021/acssuschemeng.9b02179. Acesso em: 18 abr. 2024. -
APA
Toledo, M. L., Pereira, M. M., Freire, M. G., Silva, J. P. A., Coutinho, J. A. P., & Tavares, A. P. M. (2019). Laccase Activation in Deep Eutectic Solvents. ACS Sustainable Chemistry & Engineering, 7, 11806-11814. doi:10.1021/acssuschemeng.9b02179 -
NLM
Toledo ML, Pereira MM, Freire MG, Silva JPA, Coutinho JAP, Tavares APM. Laccase Activation in Deep Eutectic Solvents [Internet]. ACS Sustainable Chemistry & Engineering. 2019 ;7 11806-11814.[citado 2024 abr. 18 ] Available from: https://doi.org/10.1021/acssuschemeng.9b02179 -
Vancouver
Toledo ML, Pereira MM, Freire MG, Silva JPA, Coutinho JAP, Tavares APM. Laccase Activation in Deep Eutectic Solvents [Internet]. ACS Sustainable Chemistry & Engineering. 2019 ;7 11806-11814.[citado 2024 abr. 18 ] Available from: https://doi.org/10.1021/acssuschemeng.9b02179 - Current Ethanol Production Requirements for the Yeast Saccharomyces cerevisiae
- Influence of aeration and substrate concentration on carotenoids and lipid production by Rhodotorula glutinis from glycerol
- Production of 5-hydroxymethylfurfural from glucose catalyzed by supported phosphotungstic acid heterogeneous catalyst
- Biodiesel production from Mucor circinelloides using ethanol and heteropolyacid in one and two-step transesterification
- Optimization rhodotorula glutinis cultivation conditions for sigle- cell-oil production from glycerol
- Direct conversion of cellulose to 5-hydroxymethylfurfural using a heterogeneous acid catalyst based on heteropolyacid.
- Utilização da sílica obtida da casca de arroz como suporte para catalisadores heteropoliácidos aplicados na reação de obtenção de biodiesel de óleo de macaúba.
- Synthesis and Application of Heterogeneous Catalysts Based on Heteropolyacids for 5-Hydroxymethylfurfural Production from Glucose
- Assessment of Nb2O5 pellet as catalyst for 5 hydroxymethylfurfural production.
- Synthesis and application of heterogeneous catalysts based on heteropolyacids for the production of 5-hydroxymethylfurfural from glucose
Informações sobre o DOI: 10.1021/acssuschemeng.9b02179 (Fonte: oaDOI API)
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
