Cloning and expression of the oligopeptide binding protein of Streptococcus mutans UA159 (2004)
- Authors:
- Autor USP: FERREIRA, RITA DE CASSIA CAFE - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Streptococcus mutans is a gram-positive pathogen responsable for thooth decay and one of the most disseminated oral pathogens. The recent publication of the complete genome of S. mutans UA159 permited the identi_cation of genes possibly involved with the pathogenicity of this bacteria. Our work aims the functional analysis of the oligopeptide uptake system (Opp), an ABC-type transport system, on the physiology and cariogenic potential of this strain. The opp genes of S. mutans are arrangend in an operon-like organization with _ve cistrons: oppA, encoding the peptide binding domain, oppB and oppC, enconding membrane proteins responsible for the uptake of substrate peptides, and oppD and oppF, enconding the ATP-binding components which provide energy for the active transport of the peptides. Among these components, OppA, confers low speci_city and high a_nity of this uptake system. In this work we report the cloning and expression of the oppA ortholog found in the genome of the S. mutans UA159 strain and production of speci_c polyclonal antibodies able to recognize the native protein. The oppA gene was ampli_ed by PCR and cloned the expression vectors pQE-30 and the pET-28a. The proteins encoded by the recombinant vectors derived from pQE-30 and pET-28a were expressed in E.coli XL1-Blue and BL21-De strains, respectively. The OppA protein encoded by the pQE-30 expression systems gave higher protein yields when compared to the pET system and most of the protein wasrecovered in the soluble fraction. The recombinant protein was successfully puri_ed after a_nity chromatography using a nickel-containing resin. The puri_ed product was recovered as a single protein used to immunize Balb/c mice. ELISA and Western blot analyses showed that sera raised in mice immunized with the recombinant protein reacted both with the recombinant protein and protein expressed by the S.mutans UA159 strain. Further experiments will target the detec
- Imprenta:
- Source:
- Título do periódico: Programa e resumos
- Conference titles: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq
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ABNT
NEPOMUCENO, R. S. L. et al. Cloning and expression of the oligopeptide binding protein of Streptococcus mutans UA159. 2004, Anais.. Caxambu: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2004. . Acesso em: 19 abr. 2024. -
APA
Nepomuceno, R. S. L., Tavares, M. B., Suzuki, C. F., Balan, A., Cai, S., Ferreira, R. de C. C., & Ferreira, L. C. de S. (2004). Cloning and expression of the oligopeptide binding protein of Streptococcus mutans UA159. In Programa e resumos. Caxambu: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Nepomuceno RSL, Tavares MB, Suzuki CF, Balan A, Cai S, Ferreira R de CC, Ferreira LC de S. Cloning and expression of the oligopeptide binding protein of Streptococcus mutans UA159. Programa e resumos. 2004 ;[citado 2024 abr. 19 ] -
Vancouver
Nepomuceno RSL, Tavares MB, Suzuki CF, Balan A, Cai S, Ferreira R de CC, Ferreira LC de S. Cloning and expression of the oligopeptide binding protein of Streptococcus mutans UA159. Programa e resumos. 2004 ;[citado 2024 abr. 19 ] - In silico analysis of polyamine uptake system and molecular cloning of potD of Streptococcus mutans
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