De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production (2018)
- Authors:
- Batista, Diego Silva
- Saldanha, Cleber Witt
- Maldaner, Joseila
- Dias, Leonardo Lucas Carnevalli
- Buselli, Reginaldo Alves Festucci
- Otoni, Wagner Campos
- Koehler, Andréa Dias
- Romanel, Elisson Antônio da Costa
- Souza, Vinicius Carius de
- Silva, Tatiane Dulcineia
- Almeida, Maíra Carolina
- Maciel, Talles Elisson F.
- Ferreira, Perácio Rafael Bueno
- Felipe, Sergio Heitor Sousa
- Batista, Diego Silva
- Autor USP: ROMANEL, ELISSON ANTÔNIO DA COSTA - EEL
- Unidade: EEL
- DOI: 10.1007/s00709-018-1322-1
- Subjects: GENÔMICA; GENÉTICA
- Keywords: Brazilian ginseng; Pfaffia glomerata; Phantom gene; Photoautotrophy; Phytoecdysteroids; Transcriptome
- Agências de fomento:
- Language: Inglês
- Abstract: Pfaffia glomerata is a medically important species because it produces the phytoecdysteroid 20-hydroxyecdysone (20-E). However, there has been no ready-to-use transcriptome data available in the literature for this plant. Here, we present de novo transcriptome sequencing of RNA from P. glomerata in order to investigate the 20-E production as well as to understand the biochemical pathway of secondary metabolites in this non-model species. We then analyze the effect of photoautotrophy on the production of 20-E genes phylogenetically identified followed by expression analysis. For this, total messenger RNA (mRNA) from leaves, stems, roots, and flowers was used to construct indexed mRNA libraries. Based on the similarity searches against plant non-redundant protein database, gene ontology, and eukaryotic orthologous groups, 164,439 transcripts were annotated. In addition, the effect of photoautotrophy in two genes putatively involved in the 20-E synthesis pathway was analyzed. The Phantom gene (CYP76C), a precursor of the route, showed increased expression in P. glomerata plants cultured under photoautotrophic conditions. This was accompanied by increased production of this metabolite indicating a putative involvement in 20-E synthesis. This work reveals that several genes in the P. glomerata transcriptome are related to secondary metabolism and stresses, that genes of the P450 family participate in the 20-E biosynthesis route, and that plants cultured under photoautotrophic conditions promote an upregulated Phantom gene and enhance the productivity of 20-E. The data will be used for future investigations of the 20-E synthesis pathway in P. glomerata while offering a better understanding of the metabolism of the species.
- Imprenta:
- Source:
- Título: Protoplasma
- ISSN: 0033-183X
- Volume/Número/Paginação/Ano: v. 256, n. , p. 601-614, 2018
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
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ABNT
BATISTA, Diego Silva et al. De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production. Protoplasma, v. 256, n. , p. 601-614, 2018Tradução . . Disponível em: https://doi.org/10.1007/s00709-018-1322-1. Acesso em: 27 dez. 2025. -
APA
Batista, D. S., Saldanha, C. W., Maldaner, J., Dias, L. L. C., Buselli, R. A. F., Otoni, W. C., et al. (2018). De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production. Protoplasma, 256( ), 601-614. doi:10.1007/s00709-018-1322-1 -
NLM
Batista DS, Saldanha CW, Maldaner J, Dias LLC, Buselli RAF, Otoni WC, Koehler AD, Romanel EA da C, Souza VC de, Silva TD, Almeida MC, Maciel TEF, Ferreira PRB, Felipe SHS. De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production [Internet]. Protoplasma. 2018 ; 256( ): 601-614.[citado 2025 dez. 27 ] Available from: https://doi.org/10.1007/s00709-018-1322-1 -
Vancouver
Batista DS, Saldanha CW, Maldaner J, Dias LLC, Buselli RAF, Otoni WC, Koehler AD, Romanel EA da C, Souza VC de, Silva TD, Almeida MC, Maciel TEF, Ferreira PRB, Felipe SHS. De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production [Internet]. Protoplasma. 2018 ; 256( ): 601-614.[citado 2025 dez. 27 ] Available from: https://doi.org/10.1007/s00709-018-1322-1 - Análise da expressão de genes BAHD em cana-de-açúcar (Saccharum spontaneum) potencialmente envolvidos na incorporação de ácidos hidroxicinâmicos
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Informações sobre o DOI: 10.1007/s00709-018-1322-1 (Fonte: oaDOI API)
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