Artigo de periodico

Expression of Melocactus glaucescens SERK1 sheds new light on the mechanism of areolar activation in cacti. (2021)

• Authors:
  • Torres-Silva, G.
  • Correia, Ludmila Nayara de Freitas
  • Koehler, Andréa Dias
  • Batista, Diego Silva
  • Faria, D. V.
  • Resende, Sheila Vitória
  • Strickler, Susan E
  • Fouracre, Jim
  • Romanel, Elisson Antonio
  • Specht, Chelsea D.
  • Otoni, Wagner Campos
• Autor USP: ROMANEL, ELISSON ANTÔNIO DA COSTA - EEL
• Unidade: EEL
• DOI: 10.1007/s11240-021-02137-9
• Subjects: CACTUS; ORGANOGÊNESE VEGETAL
• Agências de fomento:
  • Financiamento CAPES
  • Financiamento CNPq
  • Financiamento FAPEMIG
• Language: Inglês
• Abstract: Areolar activation is the most popular in vitro propagation method for cacti. Even though it is relatively simple, few estab lished protocols exist. Acquisition of a competent state has been linked to the expression of SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) during plant organogenesis. Here, cell competence acquisition and areolar activation were investigated during Melocactus glaucescens shoot organogenesis. Degenerate PCR primers and transcriptome data enabled the characterization of MgSERK1, the frst known Cactaceae SERK sequence. Phylogenetic analysis based on SERKs from 23 angiosperm species revealed elevated similarity to other SERK Dicot S1/2 sequences and identifed the corresponding SERK1 orthologs. Treated explants had the areolar region punctured three times, and shoot organogenesis was induced by exposure to 17.76 µM 6-benzyladenine and 1.34 µM 1-naphthaleneacetic acid. Shoot organogenesis was analyzed by com paring treated and non-treated explants. Anatomical examination and in situ hybridization showed that shoot organogenesis occurred via areolar activation from procambial cells in the stem cortex. Wounding of the areola region activated the axillary bud and increased the number of shoots produced per explant. In situ expression revealed the association of MgSERK1 with M. glaucescens shoot organogenesis in the areola, its adjacent regions, and the roots.
• Imprenta:
  • Publisher place: Minas Gerais
  • Date published: 2021
• Source:
  • Título do periódico: Plant Cell, Tissue and Organ Culture
  • Volume/Número/Paginação/Ano: v. 147, p. 437-451

Informações sobre o DOI: 10.1007/s11240-021-02137-9 (Fonte: oaDOI API)
• Este periódico é de assinatura
• Este artigo NÃO é de acesso aberto
  
• Cor do Acesso Aberto: closed

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How to cite

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• ABNT

  TORRES-SILVA, G. et al. Expression of Melocactus glaucescens SERK1 sheds new light on the mechanism of areolar activation in cacti. Plant Cell, Tissue and Organ Culture, v. 147, p. 437-451, 2021Tradução . . Disponível em: https://doi.org/10.1007/s11240-021-02137-9. Acesso em: 18 set. 2024.

• APA

  Torres-Silva, G., Correia, L. N. de F., Koehler, A. D., Batista, D. S., Faria, D. V., Resende, S. V., et al. (2021). Expression of Melocactus glaucescens SERK1 sheds new light on the mechanism of areolar activation in cacti. Plant Cell, Tissue and Organ Culture, 147, 437-451. doi:10.1007/s11240-021-02137-9

• NLM

  Torres-Silva G, Correia LN de F, Koehler AD, Batista DS, Faria DV, Resende SV, Strickler SE, Fouracre J, Romanel EA, Specht CD, Otoni WC. Expression of Melocactus glaucescens SERK1 sheds new light on the mechanism of areolar activation in cacti. [Internet]. Plant Cell, Tissue and Organ Culture. 2021 ; 147 437-451.[citado 2024 set. 18 ] Available from: https://doi.org/10.1007/s11240-021-02137-9

• Vancouver

  Torres-Silva G, Correia LN de F, Koehler AD, Batista DS, Faria DV, Resende SV, Strickler SE, Fouracre J, Romanel EA, Specht CD, Otoni WC. Expression of Melocactus glaucescens SERK1 sheds new light on the mechanism of areolar activation in cacti. [Internet]. Plant Cell, Tissue and Organ Culture. 2021 ; 147 437-451.[citado 2024 set. 18 ] Available from: https://doi.org/10.1007/s11240-021-02137-9

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