Using low-shear aerated and agitated bioreactor for producing two specific laccases by trametes versicolor cultures induced by 2,5-xylidine: Process development and economic analysis (2024)
- Authors:
- USP affiliated authors: MILAGRES, ADRIANE MARIA FERREIRA - EEL ; SEGATO, FERNANDO - EEL ; PRATA, ARNALDO MÁRCIO RAMALHO - EEL ; SANTOS, JÚLIO CÉSAR DOS - EEL ; FERRAZ, ANDRÉ LUIS - EEL ; SHIBUKAWA, VINÍCIUS PEREIRA - EEL ; FERNANDES, ANDRÉ JOSÉ - EEL
- Unidade: EEL
- DOI: 10.1016/j.biortech.2024.130737
- Subjects: BIOTECNOLOGIA; GENÔMICA
- Keywords: Basidiomycete; Proteome; Genome; Oxygen transfer; Enzyme cost
- Agências de fomento:
- Language: Inglês
- Abstract: Laccase isoforms from basidiomycetes exhibit a superior redox potential compared to commercially available laccases obtained from ascomycete fungi, rendering them more reactive toward mono-substituted phenols and polyphenolic compounds. However, basidiomycetes present limitations for large-scale culture in liquid media, restraining the current availability of laccases from this fungal class. To advance laccase production from basidiomycetes, a newly designed 14-L low-shear aerated and agitated bioreactor provided enzyme titers up to 23.5 IU/mL from Trametes versicolor cultures. Produced enzymes underwent ultrafiltration and LC/MS-MS characterization, revealing the predominant production of only two out of the ten laccases predicted in the T. versicolor genome. Process simulation and economic analysis using SuperPro designer® suggested that T. versicolor laccase could be produced at US$ 3.60/kIU in a 200-L/batch enterprise with attractive economic parameters and a payback period of 1.7 years. The study indicates that new bioreactors with plain design help to produce low-cost enzymes from basidiomycetes.
- Imprenta:
- Source:
- Título: Bioresource technology
- ISSN: 0960-8524
- Volume/Número/Paginação/Ano: v.401, art. n. 130737, 2024
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
FERNANDES, André J et al. Using low-shear aerated and agitated bioreactor for producing two specific laccases by trametes versicolor cultures induced by 2,5-xylidine: Process development and economic analysis. Bioresource technology, v. 401, n. art. 130737, 2024Tradução . . Disponível em: https://doi.org/10.1016/j.biortech.2024.130737. Acesso em: 01 jan. 2026. -
APA
Fernandes, A. J., Shibukawa, V. P., Prata, A. M. R., Segato, F., Santos, J. C. dos, Ferraz, A. L., & Milagres , A. M. F. (2024). Using low-shear aerated and agitated bioreactor for producing two specific laccases by trametes versicolor cultures induced by 2,5-xylidine: Process development and economic analysis. Bioresource technology, 401( art. 130737). doi:10.1016/j.biortech.2024.130737 -
NLM
Fernandes AJ, Shibukawa VP, Prata AMR, Segato F, Santos JC dos, Ferraz AL, Milagres AMF. Using low-shear aerated and agitated bioreactor for producing two specific laccases by trametes versicolor cultures induced by 2,5-xylidine: Process development and economic analysis [Internet]. Bioresource technology. 2024 ;401( art. 130737):[citado 2026 jan. 01 ] Available from: https://doi.org/10.1016/j.biortech.2024.130737 -
Vancouver
Fernandes AJ, Shibukawa VP, Prata AMR, Segato F, Santos JC dos, Ferraz AL, Milagres AMF. Using low-shear aerated and agitated bioreactor for producing two specific laccases by trametes versicolor cultures induced by 2,5-xylidine: Process development and economic analysis [Internet]. Bioresource technology. 2024 ;401( art. 130737):[citado 2026 jan. 01 ] Available from: https://doi.org/10.1016/j.biortech.2024.130737 - The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures
- An innovative concept for industrial sugarcane processing enhances polysaccharide utilization in first- and second-generation integrated biorefineries
- Techno-economic impacts of varied compositional profiles of sugarcane experimental hybrids on a biorefinery producing sugar, ethanol and electricity
- In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse
- Comparative evaluation of acid and alkaline sulfite pretreatments for enzymatic saccharification of bagasses from three different sugarcane hybrids
- Alkaline sulfite pretreatment for integrated first and second generation ethanol production: A techno-economic assessment of sugarcane hybrids
- Fate of p-hydroxycinnamates and structural characteristics of residual hemicelluloses and lignin during alkaline-sulfite chemithermomechanical pretreatment of sugarcane bagasse
- Anatomic and ultrastructural characteristics of different regions of sugar cane internodes which affect their response to alkaline-sulfite pretreatment and material recalcitrance
- Sugarcane hybrids with original low lignin contents and high field productivity are useful to reach high glucose yields from bagasse
- Topochemical characterization of sugar cane pretreated with alkaline sulfite
Informações sobre o DOI: 10.1016/j.biortech.2024.130737 (Fonte: oaDOI API)
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
