Artigo de periodico

Decyl oleate production by enzymatic esterification using Geotrichum candidum lipase immobilized on a support prepared from rice husk (2021)

• Authors:
  • Santos, Luis Fernando S.
  • Silva, Milena R. L.
  • Ferreira, Eric E. A
  • Gama, Rafaela S
  • Carvalho, Ana Karine Furtado
  • Barboza, Jayne C S
  • Luiz, Jaine Honorata Hortolan
  • Mendes, Adriano Aguiar
  • Hirata, Daniela B
• USP affiliated authors: BARBOZA, JAYNE CARLOS DE SOUZA - EEL ; CARVALHO, ANA KARINE FURTADO DE - EEL
• Unidade: EEL
• DOI: 10.1016/j.bcab.2021.102142
• Subjects: LIPASE; ESTERIFICAÇÃO; BIOTECNOLOGIA
• Keywords: Decyl oleate; Geotrichum candidum; Optimization; Enzymatic esterification
• Language: Inglês
• Abstract: The present study aimed to produce decyl oleate ester via enzymatic synthesis using a low-cost and totally homemade derivative prepared from chemically modified rice husks (phenyl-silica) and Geotrichum candidum lipase (LGC-I) produced by submerged fermentation. LGC-I was immobilized by physical adsorption on the phenyl-silica support and the effect of protein loading was then evaluated. The derivative prepared using 8.13 mg protein loading, for which an immobilization yield of 54.23% and a hydrolytic activity of 121.74 ± 1.45 U/g was obtained, was selected for subsequent esterification. The decyl oleate production process was optimized using the central composite rotational design (CCRD) for two variables: derivative concentration and reaction temperature. As regards decyl oleate production, optimal conditions are 15% (w/v) of biocatalyst at reaction temperature of 50 ◦C. A conversion of 78% was observed at 120 min of reaction. The prepared derivative was reused in 6 consecutive 120 min cycles and residual activity was maintained above 73.3%. The 1 H NMR spectroscopy revealed that only decyl oleate was produced with no signs of by-products whatsoever, also the main spectroscopic properties of the synthesized decyl oleate was observed using the IR spectrum.
• Imprenta:
  • Publisher: Elsevier
  • Publisher place: Illinois, US
  • Date published: 2021
• Source:
  • Título: Biocatalysis and agricultural biotechnology
  • ISSN: 1878-8181
  • Volume/Número/Paginação/Ano: v.36, art. 102142, p. 1-7, 2021

Informações sobre o DOI: 10.1016/j.bcab.2021.102142 (Fonte: oaDOI API)
• Este periódico é de acesso aberto
• Este artigo NÃO é de acesso aberto
  

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• ABNT

  SANTOS, Luis Fernando S. et al. Decyl oleate production by enzymatic esterification using Geotrichum candidum lipase immobilized on a support prepared from rice husk. Biocatalysis and agricultural biotechnology, v. 36, p. 1-7, 2021Tradução . . Disponível em: https://doi.org/10.1016/j.bcab.2021.102142. Acesso em: 10 fev. 2026.

• APA

  Santos, L. F. S., Silva, M. R. L., Ferreira, E. E. A., Gama, R. S., Carvalho, A. K. F., Barboza, J. C. S., et al. (2021). Decyl oleate production by enzymatic esterification using Geotrichum candidum lipase immobilized on a support prepared from rice husk. Biocatalysis and agricultural biotechnology, 36, 1-7. doi:10.1016/j.bcab.2021.102142

• NLM

  Santos LFS, Silva MRL, Ferreira EEA, Gama RS, Carvalho AKF, Barboza JCS, Luiz JHH, Mendes AA, Hirata DB. Decyl oleate production by enzymatic esterification using Geotrichum candidum lipase immobilized on a support prepared from rice husk [Internet]. Biocatalysis and agricultural biotechnology. 2021 ;36 1-7.[citado 2026 fev. 10 ] Available from: https://doi.org/10.1016/j.bcab.2021.102142

• Vancouver

  Santos LFS, Silva MRL, Ferreira EEA, Gama RS, Carvalho AKF, Barboza JCS, Luiz JHH, Mendes AA, Hirata DB. Decyl oleate production by enzymatic esterification using Geotrichum candidum lipase immobilized on a support prepared from rice husk [Internet]. Biocatalysis and agricultural biotechnology. 2021 ;36 1-7.[citado 2026 fev. 10 ] Available from: https://doi.org/10.1016/j.bcab.2021.102142

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