Characterization of magnetic particles of azocasein-iron composite for protease purification

Characterization of magnetic particles of azocasein-iron composite for protease purification (2019)

Authors:
USP affiliated authors: BENTO, HEITOR BUZETTI SIMÕES - EEL ; CARVALHO, ANA KARINE FURTADO DE - EEL
Unidade: EEL
DOI: 10.1016/j.jmmm.2019.165288
Assunto: NANOPARTÍCULAS
Keywords: Nanoparticles; Purification; Protease; Azocasein; Iron composite
Language: Inglês
Abstract: Research on magnetic particles has expanded over the years. These particles have several properties, such as low cost, fast preparation and use time, high surface area, and easy separation, which allow them to be employed in the purification of molecules, for example, enzymes. In this work, magnetic particles of azocasein-iron composite were produced, characterized and used in the purification of a microbial protease. The particle characterization was performed using the established techniques: X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). A purification rate of 11.64 was achieved by the use of magnetic particles in comparison to the crude extract. The recovery rate was very low but was higher when compared to the traditional, expensive, and slow DEAE-Sepharose column purification.
Imprenta:
- Publisher: Elsevier
- Publisher place: Campinas
- Date published: 2019
Source:
- Título: Journal of magnetism and magnetic materials
- ISSN: 0304-8853
- Volume/Número/Paginação/Ano: v.486, n. art. 165288, p. 1-4, 2019

Informações sobre o DOI: 10.1016/j.jmmm.2019.165288 (Fonte: oaDOI API)

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ABNT

AGUILAR, Jessika Gonçalves dos Santos et al. Characterization of magnetic particles of azocasein-iron composite for protease purification. Journal of magnetism and magnetic materials, v. 486, n. art. 165288, p. 1-4, 2019Tradução . . Disponível em: https://doi.org/10.1016/j.jmmm.2019.165288. Acesso em: 26 dez. 2025.
APA

Aguilar, J. G. dos S., Carvalho, A. K. F. de, Bento, H. B. S., & Sato, H. H. (2019). Characterization of magnetic particles of azocasein-iron composite for protease purification. Journal of magnetism and magnetic materials, 486( art. 165288), 1-4. doi:10.1016/j.jmmm.2019.165288
NLM

Aguilar JG dos S, Carvalho AKF de, Bento HBS, Sato HH. Characterization of magnetic particles of azocasein-iron composite for protease purification [Internet]. Journal of magnetism and magnetic materials. 2019 ;486( art. 165288): 1-4.[citado 2025 dez. 26 ] Available from: https://doi.org/10.1016/j.jmmm.2019.165288
Vancouver

Aguilar JG dos S, Carvalho AKF de, Bento HBS, Sato HH. Characterization of magnetic particles of azocasein-iron composite for protease purification [Internet]. Journal of magnetism and magnetic materials. 2019 ;486( art. 165288): 1-4.[citado 2025 dez. 26 ] Available from: https://doi.org/10.1016/j.jmmm.2019.165288