Reusing wastewater from processing to produce single-cell protein using : Optimizing of culture conditions (2023)
- Authors:
- Autor USP: LOPES, ANDRÉ MORENI - EEL
- Unidade: EEL
- DOI: 10.1002/btpr.3393
- Assunto: RESÍDUOS AGRÍCOLAS
- Keywords: Candida sorboxylosa; agro-industrial wastes; coffee wastewater (CWW); factorial fractional design; pH variation; single-cell protein (SCP)
- Agências de fomento:
- Language: Inglês
- Abstract: Coffee is a crop of significant socioeconomic importance, and the reuse of agri-food by-products and biowaste has great potential across several industries. Coffee wastewater (CWW) is a valuable resource containing essential nutrients that can be utilized by Candida sorboxylosa for single-cell protein (SCP) production. This utilization contributes to mitigating the negative impacts of agro-industrial waste. The optimization of culture conditions using the design of experiments (DoE) technique is crucial in understanding the environmental factors influencing metabolite production. In our study, the DoE technique was employed to analyze culture conditions, including room temperature, pH 8.4, agitation at 200 rpm, a headspace of 60% (v/v), and an inoculum of 0.75 DO600nm over 28-h period. This approach resulted in a remarkable SCP yield of 64.4% and dry cell weight (DCW) of 2.26 g/L. It is noteworthy that there is no literature reporting SCP production under alkaline pH conditions in yeast. Interestingly, our work demonstrated that an alkaline pH of 8.4 significantly influenced SCP production by C. sorboxylosa. The DoE technique proved to be an efficient statistical tool for optimizing culture conditions, offering several advantages, such as: (i) conducting cultures at room temperature to minimize unnecessary energy consumption; (ii) reducing the incubation time from 46 to 28 h, thereby enhancing overall productivity; (iii) achieving 1.7-fold increase in SCP yield compared to previous basal production levels.
- Imprenta:
- Publisher: Aiche
- Publisher place: Nova Iorque
- Date published: 2023
- Source:
- Título: Biotechnology Progress
- ISSN: 1520-6033
- Volume/Número/Paginação/Ano: v.39, n.5, p.1-12, art. e3393, 2023
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
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ABNT
PILLACA PULLO, Omar Santiago e LOPES, André Moreni e ESCALANTE, Waldir D. Estela. Reusing wastewater from processing to produce single-cell protein using : Optimizing of culture conditions. Biotechnology Progress, v. 39, n. 5, p. 1-12, 2023Tradução . . Disponível em: https://doi.org/10.1002/btpr.3393. Acesso em: 27 dez. 2025. -
APA
Pillaca Pullo, O. S., Lopes, A. M., & Escalante, W. D. E. (2023). Reusing wastewater from processing to produce single-cell protein using : Optimizing of culture conditions. Biotechnology Progress, 39( 5), 1-12. doi:10.1002/btpr.3393 -
NLM
Pillaca Pullo OS, Lopes AM, Escalante WDE. Reusing wastewater from processing to produce single-cell protein using : Optimizing of culture conditions [Internet]. Biotechnology Progress. 2023 ;39( 5): 1-12.[citado 2025 dez. 27 ] Available from: https://doi.org/10.1002/btpr.3393 -
Vancouver
Pillaca Pullo OS, Lopes AM, Escalante WDE. Reusing wastewater from processing to produce single-cell protein using : Optimizing of culture conditions [Internet]. Biotechnology Progress. 2023 ;39( 5): 1-12.[citado 2025 dez. 27 ] Available from: https://doi.org/10.1002/btpr.3393 - Remoção de endotoxina presente em meio fermentado contendo biomoléculas utilizando sistemas micelares de duas fases aquosas
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Informações sobre o DOI: 10.1002/btpr.3393 (Fonte: oaDOI API)
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