On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse (2019)
- Authors:
- USP affiliated authors: FERRAZ, ANDRÉ LUIS - EEL ; MILAGRES, ADRIANE MARIA FERREIRA - EEL ; SANTOS, MAIARA PAPARELE DOS - EEL ; REINOSO, FELIPE ANDRES MONTOYA - EEL
- Unidade: EEL
- DOI: 10.1016/j.bcab.2019.101081
- Subjects: CANA-DE-AÇÚCAR; BAGAÇOS; Xylanextraction
- Keywords: Luminase; Xylooligosaccharides; Sugarcane bagasse; Bacillus pumilus; Xylanase
- Agências de fomento:
- Language: Inglês
- Abstract: The purpose of this work was to investigate the enzymatic extraction of xylan using xylanases active in alkaline conditions. One on-site produced and three commercial xylanases designed to boost bleaching of pulped wood were characterized for their optimal pH, temperature and stability to compare their effects on xylan extraction from pretreated sugarcane bagasse. On-site produced xylanase was prepared from cultures of the alkaliphilic Bacillus pumilus CBMAI 0008 strain. The optimal temperatures and pH levels for all commercial xylanases were 60 °C and 6, whereas for B. pumilus xylanase, they were 50 °C and 8, respectively. B. pumilus was active up to pH 10, but it had low thermal stability. Five sugarcane substrates were prepared to contain decreasing lignin content based on alkaline-sulfite pretreatment or acid-chlorite delignification. Xylanase doses (5–100 IU/g substrate) were applied to these substrates at pH 8.0, 50 °C and a 24-h reaction. Xylan extraction yields were strongly dependent on the lignin content of the substrates. Maximal xylan extraction yields were obtained in acid-chlorite delignified substrates, reaching values of 64% and 45% for Luminase and B. pumilus xylanases, respectively. Residual (non-extractable) xylans seemed occluded by and/or complexed with residual lignin of the substrates.
- Imprenta:
- Source:
- Título: Biocatalysis and agricultural biotechnology
- ISSN: 18788181
- Volume/Número/Paginação/Ano: v.18, p.101081-, 2019
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
SANTOS, Maiara Paparele dos et al. On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse. Biocatalysis and agricultural biotechnology, v. 18, p. 101081-, 2019Tradução . . Disponível em: https://doi.org/10.1016/j.bcab.2019.101081. Acesso em: 29 dez. 2025. -
APA
Santos, M. P. dos, Reinoso, F. A. M., Távilla, V., Ferraz, A. L., & Milagres , A. M. F. (2019). On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse. Biocatalysis and agricultural biotechnology, 18, 101081-. doi:10.1016/j.bcab.2019.101081 -
NLM
Santos MP dos, Reinoso FAM, Távilla V, Ferraz AL, Milagres AMF. On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse [Internet]. Biocatalysis and agricultural biotechnology. 2019 ;18 101081-.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1016/j.bcab.2019.101081 -
Vancouver
Santos MP dos, Reinoso FAM, Távilla V, Ferraz AL, Milagres AMF. On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse [Internet]. Biocatalysis and agricultural biotechnology. 2019 ;18 101081-.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1016/j.bcab.2019.101081 - Product diversification helps to add value in the chemithermomechanical processing of sugarcane bagasse
- Fate of p-hydroxycinnamates and structural characteristics of residual hemicelluloses and lignin during alkaline-sulfite chemithermomechanical pretreatment of sugarcane bagasse
- In site produced and Commercially alkali-active xylanases compared for xylan exxtraction from sugarcane bagasse
- Comparative evaluation of acid and alkaline sulfite pretreatments for enzymatic saccharification of bagasses from three different sugarcane hybrids
- Anatomic and ultrastructural characteristics of different regions of sugar cane internodes which affect their response to alkaline-sulfite pretreatment and material recalcitrance
- Sugarcane hybrids with original low lignin contents and high field productivity are useful to reach high glucose yields from bagasse
- Topochemical characterization of sugar cane pretreated with alkaline sulfite
- Topochemical characterization of sugar cane pretreated with alkaline sulfite
- Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin
- The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures
Informações sobre o DOI: 10.1016/j.bcab.2019.101081 (Fonte: oaDOI API)
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