Mouse peritonial cells in culture connect to each other by tunneling nanotubules (2006)
- Authors:
- Autor USP: MARIANO, MARIO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Communication among cells may occur by synapses, gap junctions and cytokines. Recently, another type of cell communication has been described: tunneling nanotubules (TNT) (Immunity 43:309-318 2005). This TNT may connect two or more cells physically and propagated signal from stimulated cell to other (none stimulated) within a few seconds, even hundreds of microns away. This novel process of cell communication leads to cell activation faster than soluble signals. Herein we describe TNT communication between cells in cell cultures of adherent mouse peritoneal cells. Methods and Results: Adherent cells from peritoneal cavity were obtained and cultured for four days. Macrophages were obtained from bone marrow cell cultures conditioned by L929 medium. Cells were cultivated on glass cover slips in 24 well plates and prepared for observation by scanning electron microscopy. TNT formation was rarely observed on the surface of bone marrow derived macrophages. Conversely, TNT communication was observed in a large number in cells in cultures of peritoneal adherent cells.Communication between macrophage and B-1-like cells was observed. Conclusion: Further than TNT formation by B and T lymphocytes, NK, monocytes and dendritic cells (Immunity 43:309-318 2005), herein we described that B-1 cells also connect to others cells by TNT. Besides, adherent peritoneal cells culture, which have B-1 cells, show more TNTformation than bone marrow macrophage cultures. Considering the probable regulatory activity of B-1 cells (De Lorenzo et al, submitted), TNT formation could be the faster way of communication between them and other immune cells. The significance of TNT on B-1 cell and differences between cultures is under investigation.
- Imprenta:
- Source:
- Título: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
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ABNT
PALOS, M C e POPI, A F e MARIANO, Mário. Mouse peritonial cells in culture connect to each other by tunneling nanotubules. 2006, Anais.. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo, 2006. . Acesso em: 28 out. 2025. -
APA
Palos, M. C., Popi, A. F., & Mariano, M. (2006). Mouse peritonial cells in culture connect to each other by tunneling nanotubules. In Abstracts. São Paulo: Instituto de Ciências Biomédicas, Universidade de São Paulo. -
NLM
Palos MC, Popi AF, Mariano M. Mouse peritonial cells in culture connect to each other by tunneling nanotubules. Abstracts. 2006 ;[citado 2025 out. 28 ] -
Vancouver
Palos MC, Popi AF, Mariano M. Mouse peritonial cells in culture connect to each other by tunneling nanotubules. Abstracts. 2006 ;[citado 2025 out. 28 ] - Characterization of hybrid macrophage cell lines
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