Exploring the caulobacter crescentus sos regulon (2005)
- Authors:
- Autor USP: MENCK, CARLOS FREDERICO MARTINS - ICB
- Unidade: ICB
- Assunto: MICROBIOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Transcriptional regulation is often related to the binding of transcription factors (TFs) to specific sites of DNA (TF binding sites or TFBSs). A prominent example of this type of regulation is the E. coli SOS regulon, which is known to be involved in an "emergency" cell response to extensive damage in DNA and other stress conditions. Caulobacter crescentus is a free living alpha-proteobacterium that emerged as an important model for prokaryotic cell cycle studies. Cell division is asymetric, giving origin to two morphologically distinct daughter cells: one flagellated, motile cell and other, stalked cell. In our work, we searched for a method to define the composition of the SOS regulon of this bacterium. Methods and Results: Using a Gibbs motif sampling algorithm, we obtained a SOS TFBS model from the promoter regions of six genes known to be part of this regulon in E. coli. This motif model was seached in 5' regions of all ORFs of the C. crescentus genome. Among the genes next to the top10 scoring putative TFBSs, we found: lexA, the SOS regulon repressor; the DNA repair genes recA, uvrA, recN and dnaE (catalytic DNA polymerase); and ssb (single strand binding protein). Conclusion: With the adopted score cutoff, we were able to capture 68% of the known E. coli SOS boxes with an estimated true positive rate of 72%. Extrapolating this argument to Caulobacter crescentus genome, we could expect that about 2/3 of predicted TFBSs with thesame score cutoff would correspond to true LexA binding sites
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
PAQUOLA, Apuã César de Miranda e GALHARDO, Rodrigo da Silva e MENCK, Carlos Frederico Martins. Exploring the caulobacter crescentus sos regulon. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 27 dez. 2025. -
APA
Paquola, A. C. de M., Galhardo, R. da S., & Menck, C. F. M. (2005). Exploring the caulobacter crescentus sos regulon. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Paquola AC de M, Galhardo R da S, Menck CFM. Exploring the caulobacter crescentus sos regulon. Resumos. 2005 ;[citado 2025 dez. 27 ] -
Vancouver
Paquola AC de M, Galhardo R da S, Menck CFM. Exploring the caulobacter crescentus sos regulon. Resumos. 2005 ;[citado 2025 dez. 27 ] - Functional lentiviral vectors for xeroderma pigmentosum gene therapy
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