S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord (2005)
- Authors:
- USP affiliated author: CHADI, GERSON - ICB
- School: ICB
- Subject: ANATOMIA
- Language: Português
- Abstract: Objective: Astrocytes in the central nervous system produce S-100 protein, which is up-regulated after injury. It has been showing that the macromolecule helps to protect and to maintain neurons from neurodegeneration. Here, we analyzed S-100 expression in the contuse lesioned rat spinal cord promoted by the NYU Impactor. Methods and Results: Adult male Wistar rats (n=6) were submitted to a moderate degree of spinal cord contuse lesion. A 10g rod was dropped to the dorsal surface of the exposed spinal cord from distances of 25 mm. Another group of rats (n=4) were submitted to sham operation at T10-11 level. After 2 or 3 weeks, animals were sacrificed and their spinal cords sectioned, and processed for immunohistochemistry. S-100 immunoreactivity was detected by the avidin-biotin peroxidase technique and quantified by means of semiquantitative morphometric and microdensitometric image analysis. A strong S-100 reaction was observed bordering the lesioned site, in the region of the glial scar. Furthermore, S-100 immunoreactive cell profiles were also found to be elevated in the entire gray and white matters of the preserved tissue in cranial and caudal levels near the lesion. The S- 100 immunoreactive profiles were very similar to astrocytes showing a cell body and cell processes. S-100 reaction was seen in putative activated astrocytes. In the sham operated rats, S-100 reaction was detected only in the posterior funiculus close to the region of the laminectomy. Theresponses were seen 2 weeks after the lesion, being maintained by 3 weeks. Conclusion: The expression of S-100 in different regions of the lesioned spinal cord may be related to the formation of a glial scar close to the lesion and also to paracrine neurotrophic responses far from injury
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Place of publication: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference title: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
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ABNT
CUNHA, J. C. et al. S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 25 jun. 2022. -
APA
Cunha, J. C., Levy, B. F. A., Andrade, M. S. R., & Chadi, G. (2005). S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Cunha JC, Levy BFA, Andrade MSR, Chadi G. S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. Resumos. 2005 ;[citado 2022 jun. 25 ] -
Vancouver
Cunha JC, Levy BFA, Andrade MSR, Chadi G. S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. Resumos. 2005 ;[citado 2022 jun. 25 ] - On the presence of the bfgf immunoreactivity in the spinal cord and dorsal root ganglia of the adult rat
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