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S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord (2005)

• Authors:
  • Cunha, J. C.
  • Levy, B. F. A.
  • Andrade, M. S. R.
  • Chadi, Gerson
• Autor USP: CHADI, GERSON - ICB
• Unidade: ICB
• Assunto: ANATOMIA
• Language: Português
• Abstract: Objective: Astrocytes in the central nervous system produce S-100 protein, which is up-regulated after injury. It has been showing that the macromolecule helps to protect and to maintain neurons from neurodegeneration. Here, we analyzed S-100 expression in the contuse lesioned rat spinal cord promoted by the NYU Impactor. Methods and Results: Adult male Wistar rats (n=6) were submitted to a moderate degree of spinal cord contuse lesion. A 10g rod was dropped to the dorsal surface of the exposed spinal cord from distances of 25 mm. Another group of rats (n=4) were submitted to sham operation at T10-11 level. After 2 or 3 weeks, animals were sacrificed and their spinal cords sectioned, and processed for immunohistochemistry. S-100 immunoreactivity was detected by the avidin-biotin peroxidase technique and quantified by means of semiquantitative morphometric and microdensitometric image analysis. A strong S-100 reaction was observed bordering the lesioned site, in the region of the glial scar. Furthermore, S-100 immunoreactive cell profiles were also found to be elevated in the entire gray and white matters of the preserved tissue in cranial and caudal levels near the lesion. The S- 100 immunoreactive profiles were very similar to astrocytes showing a cell body and cell processes. S-100 reaction was seen in putative activated astrocytes. In the sham operated rats, S-100 reaction was detected only in the posterior funiculus close to the region of the laminectomy. Theresponses were seen 2 weeks after the lesion, being maintained by 3 weeks. Conclusion: The expression of S-100 in different regions of the lesioned spinal cord may be related to the formation of a glial scar close to the lesion and also to paracrine neurotrophic responses far from injury
• Imprenta:
  • Publisher: Federação de Sociedades de Biologia Experimental
  • Publisher place: Águas de Lindóia, São Paulo
  • Date published: 2005
• Source:
  • Título do periódico: Resumos
• Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE

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How to cite

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• ABNT

  CUNHA, J. C.; LEVY, B. F. A.; ANDRADE, M. S. R.; CHADI, Gerson. S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005.

• APA

  Cunha, J. C., Levy, B. F. A., Andrade, M. S. R., & Chadi, G. (2005). S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental.

• NLM

  Cunha JC, Levy BFA, Andrade MSR, Chadi G. S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. Resumos. 2005 ;

• Vancouver

  Cunha JC, Levy BFA, Andrade MSR, Chadi G. S-100 immunoreactivity is increased in white and gray matters of the contuse lesioned rat spinal cord. Resumos. 2005 ;

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