Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line

Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line (2004)

Authors:
USP affiliated authors: METZGER, MARTIN ANDREAS - ICB
Unidades: ICB
Subjects: FISIOLOGIA
Language: Inglês
Abstract: We have investigated the effects of fractions of a new type of Brazilian propolis containing phenolic acid derivatives (FRRP) on cell growth and proliferation in a human multiple myeloma cell line (RPMI 8226). The effects on cell cycle progression in the RPMI 8226 cell line were assayed with propidium iodide using DNA histograms and multiparametric flow cytometry. The inhibitory concentrations 50 (IC50) were estimated by cytostatic assay of tetrazolium (MTT) reduction. The IC50 (± S.E.M; n =3) of FRRP at 48 h exposure, was 28 ± 8 µg/ml. FRRP at a concentration of 20 µg/ml inhibited the G1/S transition of the cell cycle of the human multiple myeloma cell line, an action consistent with its cytostatic effect. Our results also revealed that mRNA expression of cyclin-dependent kinase inhibitors, p16, as assayed by RT-PCR, did not change in multiple myeloma. All procedures were performed in triplicate with appropriate controls. The chemical composition of FRRP was evaluated by High performance liquid chromatography coupled to mass detector. Our observations suggest that the FRRP from Brazilian propolis may be a powerful therapeutic drug in myeloma pathogenesis
Imprenta:
- Place of publication: Campinas
- Date published: 2004
Source:
- Título do periódico: Abstracts
Conference title: Congress of the Brazilian Society for Cell Biology

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ABNT

COSTA, Martha Silveira e; FAULHABER, Marcelo; MARCUCCI, Maria Cristina; et al. Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line. Anais.. Campinas: [s.n.], 2004.
APA

Costa, M. S. e, Faulhaber, M., Marcucci, M. C., Metzger, M. A., Bydlowski, S. P., Oliveira, A. C., & Novak, E. M. (2004). Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line. In Abstracts. Campinas.
NLM

Costa MS e, Faulhaber M, Marcucci MC, Metzger MA, Bydlowski SP, Oliveira AC, Novak EM. Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line. Abstracts. 2004 ;
Vancouver

Costa MS e, Faulhaber M, Marcucci MC, Metzger MA, Bydlowski SP, Oliveira AC, Novak EM. Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line. Abstracts. 2004 ;