Ontogenetic expression of connexins in mouse retina (2004)
- Authors:
- USP affiliated authors: BRITTO, DANIA EMI HAMASSAKI - ICB ; MORISCOT, ANSELMO SIGARI - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Português
- Abstract: Objetivo: During retinal development, gap-junction channels formed by connexins (Cx) play an essential role in the functional modeling of the organ, by mediating apoptosis signals and calcium waves activity. In this study, we determined gene expression patterns of specific connexins (Cx31.1, Cx36, Cx37 and Cx45) during mouse retinal postnatal development. Métodos e Resultados: 40 adult mice divided into 5 groups by age (P1, P5, P10, P15 and P60) were sacrificed, and their retinas were dissected for RNA isolation by using TRIzolÒ. mRNA was reversed transcribed and cDNA was amplified with a 5700 SDS Real-Time PCR machine. Data were entered into a multivariate analysis of variance (MANOVA), followed by pairwise comparisons (Tukey's HSD test). Our results indicated that neuronal connexins, such as Cx36, has low expression levels in early days of postnatal development when compared to adult (P1=42%, P. Conclusões: Connexins have different patterns of expression during retinal development. For example, Cx45 has high early levels of expression that decreases monotonically with age, suggesting for a physiological role in processes such as apoptosis signaling spreading and calcium waves activity. Otherwise, Cx36 shows an increasing level of expression during development, which could be related to the expression by differentiated specific cell types and the processing of visual signaling. Probably the specific modulation of connexins reflects their distinctphysiological role in adult and developmental retina
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2004
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
KIHARA, A. H. e MORISCOT, Anselmo Sigari e HAMASSAKI, Dânia Emi. Ontogenetic expression of connexins in mouse retina. 2004, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2004. . Acesso em: 09 nov. 2024. -
APA
Kihara, A. H., Moriscot, A. S., & Hamassaki, D. E. (2004). Ontogenetic expression of connexins in mouse retina. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Kihara AH, Moriscot AS, Hamassaki DE. Ontogenetic expression of connexins in mouse retina. Resumos. 2004 ;[citado 2024 nov. 09 ] -
Vancouver
Kihara AH, Moriscot AS, Hamassaki DE. Ontogenetic expression of connexins in mouse retina. Resumos. 2004 ;[citado 2024 nov. 09 ] - Hormônio tireoideano regula a expressão de proteínas estruturais do disco m no músculo esquelético de ratos
- Connexin 31.1 is expressed in mouse retina and modulated by dark adaptation
- O hormônio tireoideano reprime a expressão gênica de proteínas estruturais do disco M no músculo esquelético de ratos
- O hormônio tireoideano reprime a expressão da proteína M no coração de ratos.
- Connexin 31.1 is expressed in mouse retina and modulated by dark adaptation
- Spatio-temporal expression of connexins 36, 43 and 45 in developing mouse retina
- O hormônio tireoideano reprime a expressão de proteínas estruturais do disco M no coração de ratos
- O hormônio tireoideano reprime a expressão de proteínas estruturais do disco M no coração de ratos
- RT-PCR for connexin 32 from paraformaldehyde-fixed mouse retina
- RT-PCR for connexins from paraformaldehyde-fixed mouse retina
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