14.3.3 plays important role on endopeptidase 24.15 (EC 3.4.24.25) secretion (2003)
- Authors:
- Autor USP: FERRO, EMER SUAVINHO - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Português
- Abstract: The mechanism responsible for the neuropeptide-metabolizing enzyme endopeptidase 24.15 (EC 3.4.24.15; ep24.15) secretion is unknown, as the protein is encoded without a signal peptide sequence for entering the cell secretory pathway. Herein we describe an increased ep24.15 stimulated secretion upon overexpression of the 14.3.3 protein in human embryoninc kidney 293 cells (HEK293). Protein kinase (PK) A phosphorylation of ep24.15 Ser644 residue was found to be important for direct interaction with 14.3.3. Moreover, ep24.15 S644A mutation ablates the positive secretory effects of 14.3.3 overexpression. Pharmacological analyses shown that forskolin (10 µM) or phorbol ester (80 nM) increases the A23187 (7,5 µM) stimulatory effects of ep24.15 secretion; no such synergism was observed upon PKA or PKC inhibition. The ep24.15 S644A mutation also prevents the synergism between A23187 and PKA or PKC activation. Double labeling confocal microscopy analyses seems to confirm the in vivo association of ep24.15 and 14.3.3 induced by PKA or PKC activation.Therefore, these data suggest that 14.3.3-ep24.15 interactions play a key role on the mechanism of ep24.15 unconventional secretion
- Imprenta:
- Publisher: Comissão de Pesquisa do ICB/USP
- Publisher place: São Paulo
- Date published: 2003
- Source:
- Título do periódico: Resumos
- Conference titles: Congresso Instituto Ciências Biomédicas, IV
-
ABNT
CARREÑO, Flávia Regina; FERRO, Emer Suavinho; GOÑI, Camila N. 14.3.3 plays important role on endopeptidase 24.15 (EC 3.4.24.25) secretion. Anais.. São Paulo: Comissão de Pesquisa do ICB/USP, 2003. -
APA
Carreño, F. R., Ferro, E. S., & Goñi, C. N. (2003). 14.3.3 plays important role on endopeptidase 24.15 (EC 3.4.24.25) secretion. In Resumos. São Paulo: Comissão de Pesquisa do ICB/USP. -
NLM
Carreño FR, Ferro ES, Goñi CN. 14.3.3 plays important role on endopeptidase 24.15 (EC 3.4.24.25) secretion. Resumos. 2003 ; -
Vancouver
Carreño FR, Ferro ES, Goñi CN. 14.3.3 plays important role on endopeptidase 24.15 (EC 3.4.24.25) secretion. Resumos. 2003 ; - Além da conquista de Rocha e Silva. [Entrevista] por Daniela Pinto Senador
- Análise funcional da endopeptidases 24.15 (EC 3.4.24.15) e de seus mutantes com capacidade reduzida de interação à membrana plasmática
- Análise molecular da endopeptidase 24.25 (EC 3.4.24.15) nuclear
- Requisitos estruturais para a dimerização do receptor AT1 da angiotensina II
- Endopeptidase 24.15 (EC 3.4.24.15) detection in the imune system
- Peptidase activities and expression in spontaneously hypertensive rats
- Site specific determinants of protein oxidative oligomerization
- Activation of the protease thimet-oligopeptidase E.C.3.4.24.15 by cytochrome C
- Substrate binding and catalysis of the neuropeptide processing enzyme EC3.4.24.15 (EP24.15) and regulation of the neurome
- Ativação constitutiva e internalização do receptor AT1 da angiotensina II: papel da ponte dissulfeto CYS18-CYS274 e da terceira alça extracelular
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