Effects of tetramethylurea on the tertiary structure of lysozyme in water (1999)
- Authors:
- USP affiliated authors: AREAS, JOSE ALFREDO GOMES - FSP ; AREAS, ELIZABETH PINHEIRO GOMES - IQ
- Unidades: FSP; IQ
- DOI: 10.1063/1.477240
- Assunto: QUÍMICA
- Language: Inglês
- Abstract: The peculiar occurrence of pseudoplastic behaviour of protein solutions in certain organic-aqueous media has been subject of previous studies [Arêas et al., J. Coll. Interface Sci., 180 (1996), 578]. The role of the solvent in these events hasbeen shown to be determinant, suggesting that particular configurations of the solvent/water microstructure are able to induce an extensive change in the folding of proteins such as lysozyme and cytochrome c, experimentally observed. In thiswork, we present a study on the rheology, refractivity and spectroscopic (Raman scattering and ¹H and 13C nuclear magnetic resonance) behaviour of some of such binary organic-aqueous systems, as a function of the organic component mass fractionin water. Results obtained are consistent with the occurrence of a solution configurational transition at characteristic critical concentrations of the binary mixtures. The transition is apparent through several physico-chemical parametersmonitored, including Raman frequency, ¹H and 13C spin-lattice relaxation times, refractive index and viscosity. The effect has been observed for a few binary systems where protein was found to form stable solutions, including certain amides andurea derivatives, such as tetramethylurea, hexamethylphosphotriamide and dimethylformamide, some alcohols and dimethylsulfoxide. All systems presenting the effect were found to contain coexistent polar/apolar molecular domains in their organiccomponent. The effect is notobserved for systems where the organic solvent molecule lacks a significative apolar moiety, such as formamide. Results were consistent with the view of a discontinuous liquid structure for those organic/aqueousbinary mixtures
- Imprenta:
- Publisher place: Washington
- Date published: 1999
- Source:
- Título: Journal of Molecular Liquids
- Volume/Número/Paginação/Ano: n. 79, p. 45-58, 1999
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
ARÊAS, Elizabeth Pinheiro Gomes et al. Effects of tetramethylurea on the tertiary structure of lysozyme in water. Journal of Molecular Liquids, n. 79, p. 45-58, 1999Tradução . . Disponível em: https://doi.org/10.1063/1.477240. Acesso em: 12 jun. 2025. -
APA
Arêas, E. P. G., Menezes, H. H. A., Arêas, J. A. G., & Santos, P. S. (1999). Effects of tetramethylurea on the tertiary structure of lysozyme in water. Journal of Molecular Liquids, ( 79), 45-58. doi:10.1063/1.477240 -
NLM
Arêas EPG, Menezes HHA, Arêas JAG, Santos PS. Effects of tetramethylurea on the tertiary structure of lysozyme in water [Internet]. Journal of Molecular Liquids. 1999 ;( 79): 45-58.[citado 2025 jun. 12 ] Available from: https://doi.org/10.1063/1.477240 -
Vancouver
Arêas EPG, Menezes HHA, Arêas JAG, Santos PS. Effects of tetramethylurea on the tertiary structure of lysozyme in water [Internet]. Journal of Molecular Liquids. 1999 ;( 79): 45-58.[citado 2025 jun. 12 ] Available from: https://doi.org/10.1063/1.477240 - Mesoscopic segregation in binary liquid solutions: application of a model for block copolymers to non-macromolecular systems
- Low resolution NMR relaxation studies on the effects of DMSO/aqueous environment on lysozyme dynamics
- Low-resolution 'ANTPOT. 1 H' spin-spin relaxation of n-decane/water emulsions stabilized by beta-casein
- Rheology of decane/water and triglyceride/water emulsions stabilized by 'beta'-casein and sodium caseinate
- Mesoscopic segregation in binary liquid solutions: application of a model for block copolymers to non-macromolecular systems.
- Effects of extrusion on the emulsifying properties of rumen and soy protein
- Relaxação spin-spin de 'ANTPOT. 1 H' em baixa resolução de emulsões de n-decano/água estabilizadas por 'beta'-caseína
- Low resolution NMR relaxation studies on the effects of DMSO/AQUEOUS environment on lysozyme dynamics
- Espalhamento, de raios X de baixo ângulo de lisozima em tetrametilureia/água
- Hydrodynamic, optical and spectroscopic studies of some organic-aqueous binary systems
Informações sobre o DOI: 10.1063/1.477240 (Fonte: oaDOI API)
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