Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme® (2022)
- Authors:
- USP affiliated authors: SEGATO, FERNANDO - EEL ; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES - FFCLRP ; LUCAS, ROSYMAR COUTINHO DE - FMRP ; OLIVEIRA, TÁSSIO BRITO DE - FFCLRP ; PASIN, THIAGO MACHADO - FMRP ; SCARCELLA, ANA SÍLVIA DE ALMEIDA - FMRP
- Unidades: EEL; FFCLRP; FMRP
- DOI: 10.1007/s13399-020-00719-9
- Subjects: CANA-DE-AÇÚCAR; ENZIMAS HIDROLÍTICAS; BIOMASSA
- Keywords: Heterologous protein expression; Pretreatment; Sugarcane bagasse; Enzymatic hydrolysis; Biomass
- Agências de fomento:
- Language: Inglês
- Abstract: The polysaccharides in the primary plant cell wall are a renewable energy source for biofuel production. However, these polysaccharides are not readily available for bioconversion, and large enzyme sets are required to deconstruct them. Here, we aimed to improve the glucan conversion using recombinant hemicellulases and esterase as a treatment in exploded and sugarcane bagasses (SCB), followed by the addition of commercial CBH I to prevent its inhibition by hemicellulases products. A high secretion level of the recombinant enzymes was observed on SDS-PAGE. The highest activities were verified at a temperature and pH ranging from 40 to 55 °C and 4.5 to 6.0, respectively. The released reducing sugar analysis showed that all enzymes act better on SCB, with xylanase C (XynC) presenting the best activity (0.54 U/mg of protein). The high-performance liquid chromatography (HPLC) analysis demonstrated that 24 h of pretreatment was enough to reach maximum glucan conversion. The best synergy was achieved between XynC and CBH I on SCB, 1.4%. All results showed that the enzymes acted better on SCB, which can be related to the biomass composition and its molecular structure. The enzymatic pretreatment of SCB with XynC was essential to improve the glucan conversion by CBH I
- Imprenta:
- Source:
- Título: Biomass Conversion and Biorefinery
- ISSN: 2190-6815
- Volume/Número/Paginação/Ano: v. 12, p. 491-499, 2022
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
LUCAS, Rosymar Coutinho de et al. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme®. Biomass Conversion and Biorefinery, v. 12, p. 491-499, 2022Tradução . . Disponível em: https://doi.org/10.1007/s13399-020-00719-9. Acesso em: 29 dez. 2025. -
APA
Lucas, R. C. de, Oliveira, T. B. de, Lima, M. S., Pasin, T. M., Scarcella, A. S. de A., Prade, R. A., et al. (2022). Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme®. Biomass Conversion and Biorefinery, 12, 491-499. doi:10.1007/s13399-020-00719-9 -
NLM
Lucas RC de, Oliveira TB de, Lima MS, Pasin TM, Scarcella AS de A, Prade RA, Segato F, Polizeli MDLTDM. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme® [Internet]. Biomass Conversion and Biorefinery. 2022 ; 12 491-499.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1007/s13399-020-00719-9 -
Vancouver
Lucas RC de, Oliveira TB de, Lima MS, Pasin TM, Scarcella AS de A, Prade RA, Segato F, Polizeli MDLTDM. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme® [Internet]. Biomass Conversion and Biorefinery. 2022 ; 12 491-499.[citado 2025 dez. 29 ] Available from: https://doi.org/10.1007/s13399-020-00719-9 - Cold-active lytic enzymes and their applicability in the biocontrol of postharvest fungal pathogens
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Informações sobre o DOI: 10.1007/s13399-020-00719-9 (Fonte: oaDOI API)
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