Microwave-assisted enzimatic microbial biodiesel production: soybean molasses as raw material for the development of a sustainable bioprocess (2021)
- Authors:
- USP affiliated authors: CARVALHO, ANA KARINE FURTADO DE - EEL ; RÓS, PATRÍCIA CAROLINE MOLGERO DA - EEL ; MILANI, LAIS AGOSTINHO - EEL ; ZORN, SAVIENNE MARIA FIORENTINI ELERBROCK - EEL ; CONTATO, ALEX GRAÇA - FMRP
- Unidades: EEL; FMRP
- Subjects: BIODIESEL; BIOTECNOLOGIA
- Keywords: Soybean molasses; enzymatic catalysis; microbial lipids; filamentous fungus
- Agências de fomento:
- Language: Português
- Abstract: The feasibility of industrial synthesis of microbial bulk products relies on the general cost of the process. The cost of the cultivation substrate is often pointed out as a key factor in biotechnological process development. In microbial biodiesel production, the substrate represents nearly 70% of the total costs. Here, soybean molasses-based culture media was evaluated as substrate for Mucor circinelloides URM 4182 growth and lipid accumulation, and further biodiesel production of the extract lipid were performed by immobilized lipase, contributing to a fully sustainable and environmentally friendly process. Cultivations were carried out aerobically in a 1 L bioreactor at 26 ºC, 250 rpm for 120 h. Culture media consisted in soybean molasses diluted (40 g L-1 of total sugar content). Assisted-microwave extraction using ethanol as renewable solvent was applied and the obtained lipids were characterized by gas chromatography. Extracted lipids were submitted to transesterification using commercial immobilized lipase Novozym®435 at 60 ºC under microwave irradiation for 12 h. Biomass concentration of 6.5 ± 0.3 g L-1 were obtained, accumulating satisfactory lipid content (26.1 ± 0.4% wt.%). The lipids showed major composition of palmitic acid (C16:0, 31.20 wt.%) and oleic acid (C18:1, 13.72 wt.%) indicating similarity to vegetable oils and proper characteristics to be applied in biodiesel synthesis. Enzymatic produced biodiesel achieved 96.8 wt.% of ester content, which meet with international standards from ASTM and ANP. The results of this study suggested soybean molasses is a suitable raw material for microbial biodiesel production able to aid in the development of integrated industrial process in a biorefinery context, contributing to the establishment of a viable sustainable circular bioeconomy.
- Imprenta:
- Source:
- Título: Anais do 31º Congresso Brasileiro de Microbiologia
- ISSN: 2176-414X
- Volume/Número/Paginação/Ano: 1p., 2021
-
ABNT
BENTO, Heitor Buzetti Simões et al. Microwave-assisted enzimatic microbial biodiesel production: soybean molasses as raw material for the development of a sustainable bioprocess. 2021, Anais.. Online: SBM, 2021. p. 1. Disponível em: https://sbmicrobiologia.org.br/31cbm-anais/resumos/R0567-2.pdf. Acesso em: 29 dez. 2025. -
APA
Bento, H. B. S., Milani, L. A., Gonçalves, R. L. N., Conceição, J. A. B., Contato, A. G., Zorn, S. M. F. E., et al. (2021). Microwave-assisted enzimatic microbial biodiesel production: soybean molasses as raw material for the development of a sustainable bioprocess. In Anais do 31º Congresso Brasileiro de Microbiologia (p. 1). Online: SBM. Recuperado de https://sbmicrobiologia.org.br/31cbm-anais/resumos/R0567-2.pdf -
NLM
Bento HBS, Milani LA, Gonçalves RLN, Conceição JAB, Contato AG, Zorn SMFE, Da Rós PCM, Carvalho AKF de. Microwave-assisted enzimatic microbial biodiesel production: soybean molasses as raw material for the development of a sustainable bioprocess [Internet]. Anais do 31º Congresso Brasileiro de Microbiologia. 2021 ;1.[citado 2025 dez. 29 ] Available from: https://sbmicrobiologia.org.br/31cbm-anais/resumos/R0567-2.pdf -
Vancouver
Bento HBS, Milani LA, Gonçalves RLN, Conceição JAB, Contato AG, Zorn SMFE, Da Rós PCM, Carvalho AKF de. Microwave-assisted enzimatic microbial biodiesel production: soybean molasses as raw material for the development of a sustainable bioprocess [Internet]. Anais do 31º Congresso Brasileiro de Microbiologia. 2021 ;1.[citado 2025 dez. 29 ] Available from: https://sbmicrobiologia.org.br/31cbm-anais/resumos/R0567-2.pdf - Use of Fungal Mycelium as Biosupport in the Formation of Lichen-Like Structure: Recovery of Algal Grown in Sugarcane Molasses for Lipid Accumulation and Balanced Fatty Acid Profile
- Consórcio do fungo filamentoso Mucor circinelloides com duas diferentes espécies de microalgas - uma proposta eficaz para recuperação de biomassa algal
- Produção de hidrolisado de ácidos graxos essenciais por rota enzimática a partir de óleo de microalga
- Microbial lipid production from starchy sources: Use food industry waste as substrate for fungal biorefinery
- Artificial Lichens: A potential approach to harvest microalgae cells
- Enzymatic Production of Biosurfactants
- Liquens oleaginosos como alternativa sustentável para a produção de biodiesel via transesterificação in situ
- Liquens artificiais: comparação de estratégias para a colheita de biomassa microalgal
- Desenvolvimento do processo de extração de lipídios da biomassa de microalgas marinhas
- In Situ Transesterification of Microbial Biomass for Biolubricant Production Catalyzed by Heteropolyacid Supported on Niobium
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