Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance (2022)
- Authors:
- USP affiliated authors: CASTRO, HEIZIR FERREIRA DE - EEL ; CARVALHO, ANA KARINE FURTADO DE - EEL ; SOUZA, CLAUDIA REGINA FERNANDES DE - FCFRP ; FREITAS, LARISSA DE - EEL ; OLIVEIRA, WANDERLEY PEREIRA DE - FCFRP ; SILVA, TALES ALEXANDRE DA COSTA E - FCF
- Unidades: EEL; FCFRP; FCF
- DOI: 10.1016/j.cherd.2022.04.026
- Assunto: ENGENHARIA QUÍMICA
- Keywords: Spouted bed dryer; Spray dryer; Renewable carriers; Enzyme immobilization; Aroma production
- Agências de fomento:
- Language: Inglês
- Abstract: The overall stability of Candida rugosa lipase (CRL) was improved via immobilization onto low-cost eco-friendly carriers (agroindustrial wastes) followed by using spouted bed dryer or spray dryer. Lipase covalently immobilized on glutaraldehyde-activated rice husk proved the greatest performance, retaining 94.1% of the initial activity, followed by sugarcane bagasse (90.3%) and green coconut fiber (89.3%). On the other hand, the enzyme activity retention of CRL-lipase bioencapsulated in magnetic chitosan treated with glutaraldehyde or sodium tripolyphosphate ranged from 73.6% to 84.6%. The dried product moisture content, and water activities ranged from 4.1% and 6.5% and from 0.2 to 0.35, respectively. After storage for six months at 5 °C, the immobilized enzyme systems retained at least 70.0% of its initial activity (vs 18.3% retained activity for the free lipase). After ten reuse cycles, enzyme immobilized onto lignocellulosic carriers retained on average 72.7% of its initial activity. The thermostability of all immobilized derivatives was significantly improved and lipase immobilized onto rice husk showed a stabilization factor of 20.44 at 80 °C. The kinetic data showed that the CRL-lipase suffered alterations in catalytic behavior after the immobilization/drying, mainly a slight decrease in affinity to the substrate (↑KM). Among all the assessed immobilized lipase for aroma synthesis the rice husk immobilized lipase displayed the best result towards the production of isoamyl caprylate (62.40 g.L−1). In conclusion, this report reinforces the urgent need for the economic development of eco-sustainable immobilized biocatalysts to boost the use of enzymatic technology on industrial scale, and the feasibility of the association of immobilization and drying processes to improve the enzyme-quality features.
- Imprenta:
- Source:
- Título: Chemical engineering research & design
- ISSN: 02638762
- Volume/Número/Paginação/Ano: v.183, p.41-55, 2022
- Este periódico é de assinatura
- Este artigo NÃO é de acesso aberto
- Cor do Acesso Aberto: closed
-
ABNT
COSTA-SILVA, Tales Alexandre et al. Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance. Chemical engineering research & design, v. 183, p. 41-55, 2022Tradução . . Disponível em: https://doi.org/10.1016/j.cherd.2022.04.026. Acesso em: 28 dez. 2025. -
APA
Costa-Silva, T. A., Carvalho, A. K. F. de, Souza, C. R. F. de, Freitas, L., Castro, H. F. de, & Oliveira, W. P. (2022). Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance. Chemical engineering research & design, 183, 41-55. doi:10.1016/j.cherd.2022.04.026 -
NLM
Costa-Silva TA, Carvalho AKF de, Souza CRF de, Freitas L, Castro HF de, Oliveira WP. Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance [Internet]. Chemical engineering research & design. 2022 ;183 41-55.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1016/j.cherd.2022.04.026 -
Vancouver
Costa-Silva TA, Carvalho AKF de, Souza CRF de, Freitas L, Castro HF de, Oliveira WP. Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance [Internet]. Chemical engineering research & design. 2022 ;183 41-55.[citado 2025 dez. 28 ] Available from: https://doi.org/10.1016/j.cherd.2022.04.026 - Lipase immobilization on eco-friendly supports by Fluidized-Bed Technology
- Improvement of Candida rugosa lipase stability by simultaneous immobilization and drying processes: 'green' carrier and spray drying
- Avaliação de diferentes métodos de secagem de biocatalisadores obtidos pela imobilização da lipase de Candida rugosa em copolímeros magnéticos.
- Immobilized enzyme-driven value enhancement of lignocellulosic-based agricultural byproducts: application in aroma synthesis
- Enhancement lipase activity via immobilization onto chitosan beads used as seed particles during fluidized bed drying: application in butyl butyrate production
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- Penicillium citrinum whole-cells catalyst for the treatment of lipid-rich wastewater
- Critical applications of Mucor circinelloides within a biorefinery context
- Batch and continuous production of biolubricant from fusel oil and oleic acid: Lipase screening, reactor system development, and reaction optimization
Informações sobre o DOI: 10.1016/j.cherd.2022.04.026 (Fonte: oaDOI API)
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