Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars (2019)
- Authors:
- USP affiliated authors: SILVA, TATIANE DA FRANCA - EEL ; ROMANEL, ELISSON ANTÔNIO DA COSTA - EEL ; PAVANELLI, ANA CAROLINA - FM
- Unidades: EEL; FM
- DOI: 10.1186/s12870-019-2112-4
- Subjects: GENÉTICA VEGETAL; ALGODÃO
- Keywords: Biotic stress; Cotton; G. hirsutum; DCL; Dicer-like; CLRDV
- Agências de fomento:
- Language: Inglês
- Abstract: Background: Dicer-like proteins (DCLs) are essential players in RNA-silencing mechanisms, acting in gene regulation via miRNAs and in antiviral protection in plants and have also been associated to other biotic and abiotic stresses. To the best of our knowledge, despite being identified in some crops, cotton DCLs haven’t been characterized until now. In this work, we characterized the DCLs of three cotton species and analyzed their expression profiles during biotic stress. Results: As main results, 11 DCLs in the allotetraploid cotton Gossypium hirsutum, 7 and 6 in the diploid G. arboreum and G. raimondii, were identified, respectively. Among some DCLs duplications observed in these genomes, the presence of an extra DCL3 in the three cotton species were detected, which haven’t been found in others eudicots. All the DCL types identified by in silico analysis in the allotetraploid cotton genome were able to generate transcripts, as observed by gene expression analysis in distinct tissues. Based on the importance of DCLs for plant defense against virus, responses of cotton DCLs to virus infection and/or herbivore attack using two commercial cotton cultivars (cv.), one susceptible (FM966) and another resistant (DO) to polerovirus CLRDV infection, were analyzed. Both cvs. Responded differently to virus infection. At the inoculation site, the resistant cv. showed strong induction of DCL2a and b, while the susceptible cv. showed a down-regulation of these genes, wherever DCL4 expression was highly induced. A time course of DCL expression in aerial parts far from inoculation site along infection showed that DCL2b and DCL4 were repressed 24 h after infection in the susceptible cotton. As CLRDV is aphid-transmitted, herbivore attack was also checked. Opposite expression pattern of DCL2a and b and DCL4 was observed for R and S cottons, showing that aphid feeding alone may induce DCL modulation.Conclusions: Almost all the DCLs of the allotetraploide G. hirsutum cotton were found in their relative diploids. Duplications of DCL2 and DCL3 were found in the three species. All four classes of DCL responded to aphid attack and virus infection in G. hirsutum. DCLs initial responses against the virus itself and/or herbivore attack may be contributing towards virus resistance.
- Imprenta:
- Publisher place: Rio de Janeiro-RJ
- Date published: 2019
- Source:
- Título do periódico: Bmc plant biology
- ISSN: 14712229
- Volume/Número/Paginação/Ano: v.19, p.1-17, 2019
- Este periódico é de acesso aberto
- Este artigo é de acesso aberto
- URL de acesso aberto
- Cor do Acesso Aberto: gold
- Licença: cc-by
-
ABNT
MOURA, Marianna O. et al. Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars. Bmc plant biology, v. 19, p. 1-17, 2019Tradução . . Disponível em: https://doi.org/10.1186/s12870-019-2112-4. Acesso em: 19 abr. 2024. -
APA
Moura, M. O., Fausto, A. K. S., Fanelli, A., Guedes, F. A. de F., Silva, T. da F., Romanel, E. A., & Vaslin, M. F. S. (2019). Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars. Bmc plant biology, 19, 1-17. doi:10.1186/s12870-019-2112-4 -
NLM
Moura MO, Fausto AKS, Fanelli A, Guedes FA de F, Silva T da F, Romanel EA, Vaslin MFS. Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars [Internet]. Bmc plant biology. 2019 ;19 1-17.[citado 2024 abr. 19 ] Available from: https://doi.org/10.1186/s12870-019-2112-4 -
Vancouver
Moura MO, Fausto AKS, Fanelli A, Guedes FA de F, Silva T da F, Romanel EA, Vaslin MFS. Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars [Internet]. Bmc plant biology. 2019 ;19 1-17.[citado 2024 abr. 19 ] Available from: https://doi.org/10.1186/s12870-019-2112-4 - Complete genome sequences of two new virus isolates associated with cotton blue disease resistance breaking in Brazil
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Informações sobre o DOI: 10.1186/s12870-019-2112-4 (Fonte: oaDOI API)
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