Enhancement of single-cell-oil and carotenoids production by Rhodotorula glutinis in bubble column bioreactors. (2020)
- Authors:
- Autor USP: CARNEIRO, LIVIA MELO - EEL
- Unidade: EEL
- Subjects: LEVEDURAS; REATORES BIOQUÍMICOS
- Keywords: Single-cell-oil; Oleaginous yeasts; Bubble Column Bioreactor
- Agências de fomento:
- Language: Inglês
- Abstract: The present study aimed to evaluate the influence of Volumetric Oxygen Transfer Coefficient (kLa) and initial substrate concentration (S0) on the production of single- cell-oil and carotenoids by the yeast Rhodotorula glutinis NRRL-Y-12905. The experiments were carried out using face-centered 22 full factorial design to evaluate the effects of substrate concentration (70 to 250 g.L-1 ) and kLa (75 to 175 h-1 ), on the production of lipids and carotenoids. The assays were performed in Bubble Column Bioreactor, with 1.8 L capacity, at 30 °C for 120 h. The culture medium was composed by MgSO4.7H2O (1,0 g.L -1 ), KH2PO4 (20 g.L -1 ), yeast extract (3,0 g.L -1 ) and (NH4)2HPO4 (3,0 g.L -1 ) and glycerol (according to the experimental design). Within the evaluated region, the main effects of variables S0 and kLa, as well as the quadratic effect of kLa, were statistically significant at 95% confidence level on lipid and carotenoids production. The quadratic effect of kLa on lipid accumulation and the interaction effect between kLa and S0 on carotenoids production were also significant. In general, the model showed the increase in kLa and S0 favored lipid accumulation, and that a lipid accumulation greater than 10g.L-1 can be achieved for cultivation performed at S0 and kLa greater than 210 g.L-1 and 150 h-1 , respectively. For carotenoids production, the predicted maximum concentration was about 5.8 mg.L-1 , which can be achieved under kLa of 160 h-1 and S0 of 150 g.L-1 . Besides, overlapping models also made it possible to estimate a region of simultaneous optimization for both responses. Thus, cultivations performed with kLa of 150 h-1 and 220 g.L-1 of glycerol, can provide lipid and carotenoids concentrations greater than 10 g.L-1 and 5 mg.L-1 , respectively.These results contribute to establishing operational conditions that maximize single-cell oil and carotenoids production from glycerol by Rhodotorula glutinis in pneumatically agitated bioreactors.
- Source:
- Título: Brazilian Bioenergy Science & Technology Conference
- Volume/Número/Paginação/Ano: 2020
-
ABNT
PEREIRA, Vladimir dos Santos e CARNEIRO, Livia Melo e SILVA, João Paulo Alves. Enhancement of single-cell-oil and carotenoids production by Rhodotorula glutinis in bubble column bioreactors. 2020, Anais.. [S.l.]: Escola de Engenharia de Lorena, Universidade de São Paulo, 2020. Disponível em: https://bbest-biofuture.org/proceedings/b-44-enhancement-of-single-cell-oil-and-carotenoids-production-by-rhodotorula-glutinis-in-bubble-column-bioreactors/. Acesso em: 12 jun. 2025. -
APA
Pereira, V. dos S., CARNEIRO, L. M., & SILVA, J. P. A. (2020). Enhancement of single-cell-oil and carotenoids production by Rhodotorula glutinis in bubble column bioreactors. In Brazilian Bioenergy Science & Technology Conference. Escola de Engenharia de Lorena, Universidade de São Paulo. Recuperado de https://bbest-biofuture.org/proceedings/b-44-enhancement-of-single-cell-oil-and-carotenoids-production-by-rhodotorula-glutinis-in-bubble-column-bioreactors/ -
NLM
Pereira V dos S, CARNEIRO LM, SILVA JPA. Enhancement of single-cell-oil and carotenoids production by Rhodotorula glutinis in bubble column bioreactors. [Internet]. Brazilian Bioenergy Science & Technology Conference. 2020 ;[citado 2025 jun. 12 ] Available from: https://bbest-biofuture.org/proceedings/b-44-enhancement-of-single-cell-oil-and-carotenoids-production-by-rhodotorula-glutinis-in-bubble-column-bioreactors/ -
Vancouver
Pereira V dos S, CARNEIRO LM, SILVA JPA. Enhancement of single-cell-oil and carotenoids production by Rhodotorula glutinis in bubble column bioreactors. [Internet]. Brazilian Bioenergy Science & Technology Conference. 2020 ;[citado 2025 jun. 12 ] Available from: https://bbest-biofuture.org/proceedings/b-44-enhancement-of-single-cell-oil-and-carotenoids-production-by-rhodotorula-glutinis-in-bubble-column-bioreactors/ - Síntesis de biodiesel por esterificación y transesterificación simultánea de aceite de pulpa de coco Acrocomia aculeata de elevada acidez con La-ZnO como catalizador.
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