Are all dental pulp stem cells primed to differentiate into vascular endothelial cells? (2020)
- Authors:
- USP affiliated authors: VALARELLI, THAIS MARCHINI DE OLIVEIRA - FOB ; MACHADO, MARIA APARECIDA DE ANDRADE MOREIRA - FOB
- Unidade: FOB
- Subjects: CÉLULAS-TRONCO; POLPA DENTÁRIA; CÉLULAS ENDOTELIAIS; ENDOTÉLIO VASCULAR
- Agências de fomento:
- Language: Inglês
- Abstract: The purpose of this study was to evaluate if there is a sub-population of dental pulp stem cells that is uniquely primed to differentiate into vascular endothelial cells. Here, we sorted SHED or DPSC according to expression levels of VEGFR1 and exposed them to one of the following conditions: 1) Control medium; 2) Endothelial differentiation medium - Endothelial Growth Medium (EGM) 2-MV supplemented with 50 ng/mL rhVEGF in presence of 0 or 25 μg/ml Bevacizumab. We evaluated cell proliferation and endothelial differentiation potential in vitro through the: Sulforhodamine-B (SRB) assay, Capillary Sprouting Assay, RT-PCR, Western Blot, and Immunofluorescence. We also transplanted VEGFR1-sorted cells into immunodeficient mice and evaluated the impact of VEGFR1 expression levels on microvessel density by histology and immunohistochemistry. We confirmed that dental pulp stem cells differentiate into endothelial cells by increased expression of endothelial cell markers and by capillary sprouting formation upon exposure to endothelial differentiation medium. VEGFR1low dental pulp stem cells proliferated quicker than VEGFR1high cells, indicating their preferential selfrenewal. On the other hand, VEGFR1high cells are more prone to vasculogenic differentiation, as they generated more capillary sprouts in vitro and more microvessels in vivo than VEGFR1low cells. These data demonstrate that stem cells of the dental pulp have an unique sub-population of cells defined by high VEGFR1 expression that are primed to differentiate into vascular endothelial cells.
- Imprenta:
- Source:
- Título: Brazilian Oral Research
- Volume/Número/Paginação/Ano: 1807-3107
- Conference titles: Sociedade Brasileira de Pesquisa Odontológica - SBPqO - Virtual Annual Meeting
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ABNT
BERGAMO, M. T. O. P. et al. Are all dental pulp stem cells primed to differentiate into vascular endothelial cells?. Brazilian Oral Research. São Paulo: Faculdade de Odontologia de Bauru, Universidade de São Paulo. . Acesso em: 06 out. 2024. , 2020 -
APA
Bergamo, M. T. O. P., Zhang, Z., Oliveira, T. M. de, Machado, M. A. A. M., & Nör, J. E. (2020). Are all dental pulp stem cells primed to differentiate into vascular endothelial cells? Brazilian Oral Research. São Paulo: Faculdade de Odontologia de Bauru, Universidade de São Paulo. -
NLM
Bergamo MTOP, Zhang Z, Oliveira TM de, Machado MAAM, Nör JE. Are all dental pulp stem cells primed to differentiate into vascular endothelial cells? Brazilian Oral Research. 2020 ;[citado 2024 out. 06 ] -
Vancouver
Bergamo MTOP, Zhang Z, Oliveira TM de, Machado MAAM, Nör JE. Are all dental pulp stem cells primed to differentiate into vascular endothelial cells? Brazilian Oral Research. 2020 ;[citado 2024 out. 06 ] - Importância dos programas de educação e motivação para saúde bucal em escolas: relato de experiência
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