Silencing of STI1 gene in murine embryonic stem cells to evaluate pluripotency markers (2013)
- Authors:
- Autor USP: LOPES, MARILENE HOHMUTH - ICB
- Unidade: ICB
- Subjects: BIOLOGIA CELULAR; BIOLOGIA DO DESENVOLVIMENTO; CÉLULAS-TRONCO (EMBRIOLOGIA)
- Language: Português
- Abstract: Introdução Several studies have shown that the protein Stress Inducible Protein 1 (STI1) participates in many biological processes, such as, the self-renewal and proliferation of neuronal stem-cells derived from fetal mouse telenchephalon. In attempt to understand the physiological role of STI1 in mammalian development, constitutive knockout mice were generated. Remarkably, the deletion of STI1 gene in homozygosis is lethal and the most of knockout embryos remains in blastocyst stage and few are able to reach the embryonic day 10, indicating that STI1 plays an essential role on mammalian embryogenesis. It is known that the pluripotent state of embryonic stem cells (ESCs) is orchestrated by a series of transcription factor, including: Oct3/4 (Pou5f1) and Sox2 (sex determining region 2), both acting on the inner cell mass (ICM) maintenance of blastocyst stage and gene expression, such as Fgf4 (Fibroblast Growth Factor 4), which has a role on the Fgf/Mek/Erk signaling pathway; and Nanog, which activity is related with the epiblast maintenance and its expression can sustain the self-renewal in murine ESCs in the absence of LIF (Leukemia Inhibitor Factor). Objetivos The main objective of this study is to evaluate the role of STI1 on the regulation of pluripotency of murine embryonic stem-cells;Métodos To achieve the objective, a murine embryonic stem-cell lineage (TG2A) will be used to modulate STI1 expression through interference RNA. The expression of pluripotency markers will be analyzed by qRT-PCR, immunofluorescence and western blotting in TG2A population expressing different STI1 levels. Resultados To silence STI1 gene in TG2A lineage, firstly we standardized the lipofection procedure using reporter genes (dsRED and GFP) and we obtained around 40 % of transfection efficiency. Thereafter we transfected TG2A cell line with three distinct siRNA sequences targetting STI1 mRNA and aproximatelly 40% of cells had STI1 gene silenced as assessed by western blotting and immunofluorescence. Conclusão A transient population of embryonic stem cells, TG2A, expressing diferent leves of STI1 was generated and will be used in qRT-PCR and Western blloting assays to evaluate whether STI1 levels could modulate the expression of pluripotency markers and consequently regulate the embryonic stem cells maintanence.
- Imprenta:
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental (FeSBE)
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ABNT
FARIA , F. P. M. et al. Silencing of STI1 gene in murine embryonic stem cells to evaluate pluripotency markers. 2013, Anais.. Caxambu: FeSBE, 2013. . Acesso em: 13 fev. 2026. -
APA
Faria , F. P. M., Romero, A. A., Santos, T. G., & Lopes, M. H. (2013). Silencing of STI1 gene in murine embryonic stem cells to evaluate pluripotency markers. In Resumos. Caxambu: FeSBE. -
NLM
Faria FPM, Romero AA, Santos TG, Lopes MH. Silencing of STI1 gene in murine embryonic stem cells to evaluate pluripotency markers. Resumos. 2013 ;[citado 2026 fev. 13 ] -
Vancouver
Faria FPM, Romero AA, Santos TG, Lopes MH. Silencing of STI1 gene in murine embryonic stem cells to evaluate pluripotency markers. Resumos. 2013 ;[citado 2026 fev. 13 ] - Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain
- Evidence of extracellular vesicles biogenesis and release in mouse embryonic stem cells
- Engagement of cellular prion protein with the co-chaperone Hsp70/90 organizing protein regulates the proliferation of glioblastoma stem-like cells
- Regulation of stress-inducible phosphoprotein 1 nuclear retention by protein inhibitor of activated STAT PIAS1
- Prion potency in stem cells biology
- Targeting prion protein interactions in cancer
- The growth of glioblastoma orthotopic xenografts in nudemice is directly correlated with impaired object recognition memory
- The unconventional secretion of stress-inducible protein 1 by a heterogeneous population of extracellular vesicles
- Nuclear unphosphorylated STAT3 correlates with a worse prognosis in human glioblastoma
- STI1 antagonizes cytoskeleton collapse mediated by small GTPase Rnd1 and regulates neurite growth
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