Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain (2013)
- Authors:
- Autor USP: LOPES, MARILENE HOHMUTH - ICB
- Unidade: ICB
- Subjects: BIOLOGIA CELULAR; BIOLOGIA DO DESENVOLVIMENTO; CELULAS-TRONCO; PLASTICIDADE NEURAL; SISTEMA NERVOSO CENTRAL
- Language: Português
- Abstract: Introdução The ocurrence of neurogenesis in adult brain was disbelieved for several years. Nowadays, it is recognized the existence of neurogenic niches in mammalian adult brain, sites where new neurons are generated throughout life, being the best studied the ventricular-subventricular zone (V-SVZ) and subgranular zone (SGZ). These niches harboring adult multipotent neural stem cells (adNSCs) that give rise to new neurons and glial cells. While science has evolved into acceptance of neurogenesis, important studies have reported the cellular prion protein (PrPC), a membrane glycoprotein highly expressed in the central nervous system (CNS) and widely conserved during evolution, as an important factor in neurogenesis and biology of adNSCs. Steele and colleagues reported the PrPC as important factor in neurogenesis during development and adulthood. Moreover, our group identified as PrPC ligand, the co-chaperone called stress-inducible protein one (STI1), which interaction promotes neuritogenesis, neuroprotection, formation of short-term memory (STM), long-term memory (LTM) consolidation and self-renewal of fetal neural stem cells. Given these findings, it becomes important to know if the PrPC-STI1 complex also acts similarly in adNSCs. This study provide a better understanding of mechanisms that mediate neurogenesis and plasticity in the adult CNS, as well as novel strategies for the treatment of neurological disorders. Objetivos To evaluate the role of the PrPC-STI1 complex in the self-renewal, proliferation and differentiation of adNSCs from V-SVZ murine brain.Métodos Neurosphere primary cultures of adNSCs derived from the V-SVZ from wild-type adult animals ( PrPn +/+) or knockout ( Prpn0/0) for PrPC have been performed in the presence of absence of recombinant STI1 in self-renewal or proliferation assays. This study was approved by Animal Ethic Committe from A.C. Camargo Hospital (protocol 025/08). Resultados Preliminary results indicate that STI1 is able to promote self-renewal and proliferation in murine adNSCs in a PrPC-dependent manner. Conclusão Additional experiments should be performed to confirm the participation of PrPC-STI1 complex upon proliferation and self-renewal of adNSCs.
- Imprenta:
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental (FeSBE)
-
ABNT
DA SILVA, C. M. C. et al. Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. 2013, Anais.. Caxambu: FeSBE, 2013. . Acesso em: 04 ago. 2025. -
APA
Da Silva, C. M. C., Santos, T. G., Martins, V. R., & Lopes, M. H. (2013). Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. In Resumos. Caxambu: FeSBE. -
NLM
Da Silva CMC, Santos TG, Martins VR, Lopes MH. Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. Resumos. 2013 ;[citado 2025 ago. 04 ] -
Vancouver
Da Silva CMC, Santos TG, Martins VR, Lopes MH. Role of the interaction between prion protein and stress-inducible protein 1 in the self-renewal in vitro of neural precursors from adult brain. Resumos. 2013 ;[citado 2025 ago. 04 ] - STI1 antagonizes cytoskeleton collapse mediated by small GTPase Rnd1 and regulates neurite growth
- Evidence of extracellular vesicles biogenesis and release in mouse embryonic stem cells
- Silencing of STI1 gene in murine embryonic stem cells to evaluate pluripotency markers
- Nuclear unphosphorylated STAT3 correlates with a worse prognosis in human glioblastoma
- The unconventional secretion of stress-inducible protein 1 by a heterogeneous population of extracellular vesicles
- The growth of glioblastoma orthotopic xenografts in nudemice is directly correlated with impaired object recognition memory
- Engagement of cellular prion protein with the co-chaperone Hsp70/90 organizing protein regulates the proliferation of glioblastoma stem-like cells
- Modulation of hippocampal neuronal resilience during aging by the Hsp70/Hsp90 co‐chaperone STI1
- Proteina prion e seus ligantes: determinantes moleculares na biologia de células-tronco e tumores cerebrais
- Extracellular vesicles: decoding a new language for cellular communication in early embryonic development
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
