Toxic effects of mycophenolic Acid on ARPE-19 and human muller cells in culture (2010)
- Authors:
- Autor USP: VENTURA, DORA SELMA FIX - IP
- Unidade: IP
- Subjects: CULTURA DE CÉLULAS; CÉLULAS MORTAS; FÁRMACOS (TOXICIDADE)
- Language: Inglês
- Abstract: Purpose:To evaluate the effects of MPA over two distinct human retinal cell lines in vitro: human retinal pigment epithelium (ARPE-19) and Human Muller Cells (type MIO M-1), and to determine a safety profile of MPA in vitro. Methods:ARPE-19 cells (ATCC, Manassas, VA) and MIO M-1 cells (Dept. of Cell Biology of the University College, London, UK) were treated with 25, 50 and 100 µg/ml of Mycophenolate mofetil (Cellcept, Roche, Nutley, NJ) for 24 hours. The powder was mixed and diluted into culture media in order to achieve the desired concentration. Safety was evaluated by trypan blue dye-exclusion cell viability assay, JC-1 mitochondrial membrane potential (m) assay, and caspase-3/7 apoptosis related assay. Results:Both cell lines showed a significant reduction in cell viability at the highest dose after 24 hours (ARPE-19 cells: 69.75 ± 1.06%, 92.10 ± 1.84% and 91.90 ± 1.83% for 100, 50 or 25µg/ml, respectively versus 96.10 ± 0.42% for untreated controls; MIO M-1 cells: 74.80 ± 1.84%; 87.00 ± 2.26% and 89.02 ± 0.99% for 100, 50 or 25µg/ml, respectively and 90.35 ± 0.64% for untreated controls, p<0.001 for both cell lines). The caspase 3/7 apoptosis related assay showed a statistically higher activity for both cell lines at the 100µg/ml concentration (ARPE-19: 14248.38 ± 1833.37, 5418.44 ± 2583.94, and 3274.44 ± 1458.62 msi for 100, 50 and 25µg/ml respectively, versus 2705.03 ± 488.26 msi for untreated controls, p<0.001; MIOM-1 cells: 14405.03 ± 1339.51, 5649.29 ± 1269.98, and 4251.13 ± 3211.69 msi for 100, 50 and 25µg/ml respectively, versus 2593.33 ± 937.53 msi for untreated controls, p<0.05). The JC-1 mitochondrial membrane potential (m) did not show statistical differences for both cell lines testedConclusions:Doses of MPA up to 50µg/ml seem to be safe in vitro. This data must be validated by experimental models in vivo in order to confirm its safety for clinical use. Future experiments with autoimmune models should be performed as well to determine if local intraocular applications of MPA are effective in controlling inflammation
- Imprenta:
- Publisher: Association for Research in Vision and Ophthalmology
- Publisher place: Lauderdale, Florida
- Date published: 2010
- Source:
- Título do periódico: Anais
- Conference titles: Annual Meeting of the Association for Research in Vision and Ophthalmology
-
ABNT
ZACHARIAS, L. C et al. Toxic effects of mycophenolic Acid on ARPE-19 and human muller cells in culture. 2010, Anais.. Lauderdale, Florida: Association for Research in Vision and Ophthalmology, 2010. Disponível em: http://abstracts.iovs.org/cgi/content/abstract/51/5/3322?maxtoshow=&hits=10&RESULTFORMAT=1&author1=Ventura&andorexacttitle=and&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&volume=51&resourcetype=HWCIT,HWELTR. Acesso em: 27 abr. 2024. -
APA
Zacharias, L. C., Damico, F. M., Ventura, D. S. F., Gasparin, F., Kenney, M. C., & Kuppermann, B. D. (2010). Toxic effects of mycophenolic Acid on ARPE-19 and human muller cells in culture. In Anais. Lauderdale, Florida: Association for Research in Vision and Ophthalmology. Recuperado de http://abstracts.iovs.org/cgi/content/abstract/51/5/3322?maxtoshow=&hits=10&RESULTFORMAT=1&author1=Ventura&andorexacttitle=and&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&volume=51&resourcetype=HWCIT,HWELTR -
NLM
Zacharias LC, Damico FM, Ventura DSF, Gasparin F, Kenney MC, Kuppermann BD. Toxic effects of mycophenolic Acid on ARPE-19 and human muller cells in culture [Internet]. Anais. 2010 ;[citado 2024 abr. 27 ] Available from: http://abstracts.iovs.org/cgi/content/abstract/51/5/3322?maxtoshow=&hits=10&RESULTFORMAT=1&author1=Ventura&andorexacttitle=and&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&volume=51&resourcetype=HWCIT,HWELTR -
Vancouver
Zacharias LC, Damico FM, Ventura DSF, Gasparin F, Kenney MC, Kuppermann BD. Toxic effects of mycophenolic Acid on ARPE-19 and human muller cells in culture [Internet]. Anais. 2010 ;[citado 2024 abr. 27 ] Available from: http://abstracts.iovs.org/cgi/content/abstract/51/5/3322?maxtoshow=&hits=10&RESULTFORMAT=1&author1=Ventura&andorexacttitle=and&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&volume=51&resourcetype=HWCIT,HWELTR - Alterações neuropsicológicas em pacientes com esclerose múltipla tipo remitente-recorrente
- Morphological and physiological characteristics of photoreceptors of the bee compound eye
- Comparação de métodos de discriminação de cores em melipona quadrifasciata
- Improved spectral sensitivity measurement in bee photoreceptors made with the constant response method
- Marking and recording of monopolar cells in the bee lamina
- Somacao temporal e duracao critica - comparacao de metodos para a sua medicao em celulas retinulares de insetos
- Ritmo circadiano de alteracoes morfologicas no olho composto da sauva atta sexdens ( hymenoptera formicidae )
- Evidencias ultra-estruturais para a funcao de fotorreceptores cerebrais extra-oculare em insetos
- Distribuicao das goticulas de oleo no beija-flor, periquito australiano e codorna
- Discriminacao de padroes por condicionamento operante no beija-flor eupetomena macroura macroura
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
