Behavioral and immunological response alterations induced by cohabitation with a melanoma-bearer C57BL/6 mouse cage mate (2006)
- Autor USP: BARBUTO, JOSE ALEXANDRE MARZAGAO - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction and Objectives: Caregiving may be a disturbing signal to both, the Nervous and the Immune systems. Though there are many clinical reports of caregivers' increased susceptibilty to disease, few animal models address this issue. Herein, we explore a modification of a murine Ehlich Ascites Tumor (EAT) model that uses, instead, the murine melanoma B16F10. These cells metastasize early to the lungs, but do not induce ascites formation in the animals. We investigated if this could affect the cage-mate, as the cohabitation with an EAT-bearer has been proven to do. For this, we analyzed the behavior and the delayed type hipersensitivity (DTH) response to ovalbumin (OVA) inoculated after the cohabitation with a B16F10 bearer cage-mate in C57BL/6 (B6) mice. Methods and Results: The present study evaluated the behavioral and immunological alterations in healthy mice that cohabitated (SC) or not (HC) with a 20-day B16F10 bearer. Random pairs of female B6 mice, 4-8 weeks old, were kept in cages for 5 days before the injection of 1 x 106 B16F10 viable cells or with vehicle into the footpad. On day 20 (tumors were detected after 10-12 days), healthy cage-mates were submitted to behavioral analysis or sensitized with OVA (50 µg in Complete Freund's Adjuvant).Sensitized animals were challenged 8 days later with OVA and their footpad increase followed for 72h. Cohabitation with a sick-cage mate induced significant increases (p<0.05) inexploration time (HC: 2013 ± 416.3 vs SC: 2503 ± 356.3) and mean velocity (HC: 6.72 ± 1.39 vs SC: 8.35 ± 1.19) at the open field, and decreases (p<0.05) in both number of entries (HC: 15.17 ± 5.98 vs SC: 7.38 ± 1.60) and time of exploration (HC: 11.67 ± 4.27 vs SC: 6.81 ± 1.04) at the open-arms in the elevated plus-maze apparatus. Although the initial inflammatory response was undisturbed in both SC and HC animals, SC mice were not able to establish a DTH reaction to OVA as the control animals did. Conclusion: Altogether our results strongly suggest that cohabitation with a 20-day melanoma bearer induces behavioral alterations and affects the establishment of an immune response.
- Título do periódico: Abstracts
- Conference titles: Meeting of the Brazilian Society for Immunology
ABNTTOMIYOSHI, M Y; STANKEVICIUS, D; MASSOCO, C O; PALERMO-NETO, João; BARBUTO, José Alexandre Marzagão. Behavioral and immunological response alterations induced by cohabitation with a melanoma-bearer C57BL/6 mouse cage mate. Anais.. São Paulo: [s.n.], 2006.
APATomiyoshi, M. Y., Stankevicius, D., Massoco, C. O., Palermo-Neto, J., & Barbuto, J. A. M. (2006). Behavioral and immunological response alterations induced by cohabitation with a melanoma-bearer C57BL/6 mouse cage mate. In Abstracts. São Paulo.
NLMTomiyoshi MY, Stankevicius D, Massoco CO, Palermo-Neto J, Barbuto JAM. Behavioral and immunological response alterations induced by cohabitation with a melanoma-bearer C57BL/6 mouse cage mate. Abstracts. 2006 ;
VancouverTomiyoshi MY, Stankevicius D, Massoco CO, Palermo-Neto J, Barbuto JAM. Behavioral and immunological response alterations induced by cohabitation with a melanoma-bearer C57BL/6 mouse cage mate. Abstracts. 2006 ;
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