Development and characterization of murine models of T lymphoma to investigate the impact of oncogene expression (2005)
- Authors:
- Autor USP: MENDES, JOAO GUSTAVO PESSINI AMARANTE - ICB
- Unidade: ICB
- Assunto: IMUNOLOGIA
- Language: Inglês
- Abstract: Introduction: Many studies in the area of cancer have been developed, however the understanding of tumorigenesis and the mechanisms of immune response against tumors is still far from being elucidated. RMA-S cells is a mutant, TAP-deficient lymphoma line derived from RMA cells. In contrast to the latter, RMA-S cells are deficient in the expression of MHC class I and, therefore, are good targets for NK cells. Aim: We are interested in using this pair of lymphoma cell lines to establish a mouse-based model that can be used to a) understand and manipulate CD8 T lymphocyte versus NK cell immune responses to tumors; b) investigate the effect of ectopic expression of certain oncogenes, tumor suppressor genes and molecules related to cell death and migration in tumor behavior in vivo. Methods: Bicistronic retroviral vector containing a multiple cloning site and the egfp gene under control of the same promoter was used to develop RMA.EGFP and RMA-S.EGFP cells. To develop our experimental model, eight to twelve week-old female WT and athymic C57Bl/6 mice were iv injected with different cell concentrations and survival curves were produced. In addition, tumor spread and infiltration was analyzed by histopathology in different organs. Results and Conclusion: First, we characterized the proliferation and apoptosis resistance to chemotherapeutic drugs in vitro, and observed no difference between the two cell lines. When injected in vivo, RMA.EGFP cells inducedprogressive paraplegia, while RMA-S.EGFP promoted important ascites and liver metastasis. The symptoms appeared 15-20 days post injection of ≥104 cells and the death was 30-40 days post injection, in average. As expected, we found both tumor cells in the spleen. Concentrations of 103 cells do not induced pathology or death of the WT mice. Importantly, when these mice were re-injected with 106 RMA.EGFP, they were more resistant to paraplegia and death
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
PANTALEÃO, Claudia et al. Development and characterization of murine models of T lymphoma to investigate the impact of oncogene expression. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 12 fev. 2026. -
APA
Pantaleão, C., Jacysyn, J. F., Castro, F. A., & Amarante-Mendes, J. G. P. (2005). Development and characterization of murine models of T lymphoma to investigate the impact of oncogene expression. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Pantaleão C, Jacysyn JF, Castro FA, Amarante-Mendes JGP. Development and characterization of murine models of T lymphoma to investigate the impact of oncogene expression. Resumos. 2005 ;[citado 2026 fev. 12 ] -
Vancouver
Pantaleão C, Jacysyn JF, Castro FA, Amarante-Mendes JGP. Development and characterization of murine models of T lymphoma to investigate the impact of oncogene expression. Resumos. 2005 ;[citado 2026 fev. 12 ] - Therapeutic applications of TRAIL receptor agonists in cancer and beyond
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