Can nuclear encoded genes lacking a bipartite signal sequence be transported to the apicoplast in apicomplexa? (2005)
- Authors:
- Autor USP: OBANDO, HERNANDO A. D. P. - ICB
- Unidade: ICB
- Assunto: PARASITOLOGIA
- Language: Inglês
- Abstract: Parasitic protozoa of Apicomplexa possess a distinct and essential organelle of algal origin termed the apicoplast. As it is not present in the mammalian host cells, the apicoplast has become the target of intense research to look for alternative control strategies against human diseases such as malaria and toxoplasmosis. Its genes are noncoding or code for ribosomal proteins and, like other endosymbiontic genomes, most of its genes were transferred to the nuclear genome. Thus, an in silico survey based on nuclear genes containing the bipartite signal sequence, identified 466 genes (~ 10% of the nuclear genome) (Foth et al. 2003. Science 299:705). Of notice, reverse genetics approaches have validated that some of these genes are indeed transported to the apicoplast. A different approach was pursued to predict genes encoding apicoplast proteins by using the trasncriptome analysis of the asexual blood stages of Plasmodium falciparum at 1h resolution (Bozdech et al. 2003. PLoS 1:1). Based on these results, a probabilistic genetic network of the apicoplast (api-PGN) was constructed (Barrera et al. Critical Assessment of Microarray Data Analysis, in press; www.camda.duke.edu/camda/camda04). Interestingly, many nuclear encoded genes that lack a bipartite signal and that have homologous plastid genes in Arabidopsis thaliana were predicted by this new tool. We selected some of them, searched for their homologous in Toxoplasma gondii and constructed expression vectorscontaining some of such genes in fusion with GFP or myc-epitope in order to validate the api-PGN network and to determine their sub-cellular localization in T. gondii. Results on these experiments will be presented
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
SENDODA, Andrea Mayumi Chin et al. Can nuclear encoded genes lacking a bipartite signal sequence be transported to the apicoplast in apicomplexa? 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 10 jan. 2026. -
APA
Sendoda, A. M. C., Martins, D., Marcondes, R. C., Pereira, B. C., Barrera, J., Del Portillo, H. A., & Fernandez-Becerra, C. (2005). Can nuclear encoded genes lacking a bipartite signal sequence be transported to the apicoplast in apicomplexa? In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Sendoda AMC, Martins D, Marcondes RC, Pereira BC, Barrera J, Del Portillo HA, Fernandez-Becerra C. Can nuclear encoded genes lacking a bipartite signal sequence be transported to the apicoplast in apicomplexa? Resumos. 2005 ;[citado 2026 jan. 10 ] -
Vancouver
Sendoda AMC, Martins D, Marcondes RC, Pereira BC, Barrera J, Del Portillo HA, Fernandez-Becerra C. Can nuclear encoded genes lacking a bipartite signal sequence be transported to the apicoplast in apicomplexa? Resumos. 2005 ;[citado 2026 jan. 10 ] - Mining the malaria transcriptome
- Primary structure of the merozoite surface antigen 1 of plasmodium vivax reveals sequences conserved between different plasmodium species
- Luminescence-based assay for antimalarial drug screening
- Comparison of introns in a cdc-2-homologous gene within a number of plasmodium species
- Molecular characterization of the cell cycle control cdc2 gene of plasmodium vivax
- Longevity of naturally acquired antibody responses to the n-and c-terminal regions of plasmodium vivax merozoite surface protein 1
- Characterization of the Cdc2-related kinase 2 gene from Plasmodium knowlesi and P. berghei
- Plasmodium vivax: primary structure and allele polymorphism of the PVMDR1 gene among isolates from Brazil, papua and monkey-adapted strains
- Plasmodium vivax: allele variants of the mdr1 gene do not associate with chloroquine resistance among isolates from Brazil, Papua, and monkey-adapted strains
- Evidence for different mechanisms of chloroquine in two plasmodium species that cause human malaria
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
