Luminescence-based assay for antimalarial drug screening (2005)
- Authors:
- Autor USP: OBANDO, HERNANDO A. D. P. - ICB
- Unidade: ICB
- Assunto: PARASITOLOGIA
- Language: Inglês
- Abstract: The rapid spread of multidrug resistance in the most deadly human malaria parasite, Plasmodium falciparum, has motivated worldwide efforts to look for new antimalarial drugs and to develop high-throughput screenings for these new compounds. Indeed, the microscopic test based on morphological estimation of parasite growth cannot be use for massive screening; yet, it remains a "gold-standard" to validate new tests. The first highthroughput test to evaluate parasite sensitivity was developed based on the measurement of the metabolic activity by radioactive marker, [3H]-labelled hypoxanthine. Although this method is highly accurate and precise, it poses the problems of handling radioisotopes. To overcome this limitation, two other non-isotopic tests that measure parasite antigens by immune detection, namely lactate dehydrogenase, pLDH and Histidine-Rich Protein II, HRP2, were developedAlthough these methods have been validated through comparisons with the microscopic and isotope methods, the present cost-per-reaction limits their value in large-scale studies. More recently, a new test based on SYBER Green I-based fluorescence has been developed. Although it offers several advantages as low cost and easily handling, SYBER green is an intercalating agent that will detect indiscriminately any contaminant DNA. We report here the development of an alternative, luminescence-based in vitro assay to screen new drugs againstP. falciparum. The assay is based on a transgenic P. falciparum parasite clone stably and highly expressing luciferase throughout the asexual blood stages. Thus, after drug exposure the parasite survival is evaluated by remaining luciferase activity. Preliminary results demonstrated high sensitivity and reproducibility of the test when compared to the isotopic method suggesting that this assay could be convenient for high-throughput screening of antimalarial drugs against P. falciparum. In addition it is simple, fast and inexpensive. Further validations of this assay will be presented
- Imprenta:
- Publisher: Comissão de Cultura e Extensão Universitária do ICB/USP
- Publisher place: São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Congresso do Instituto de Ciências Biomédicas
-
ABNT
YAMAMOTO, M. M. e DEL PORTILLO, Hernando Antonio. Luminescence-based assay for antimalarial drug screening. 2005, Anais.. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005. . Acesso em: 10 jan. 2026. -
APA
Yamamoto, M. M., & Del Portillo, H. A. (2005). Luminescence-based assay for antimalarial drug screening. In Resumos. São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP. -
NLM
Yamamoto MM, Del Portillo HA. Luminescence-based assay for antimalarial drug screening. Resumos. 2005 ;[citado 2026 jan. 10 ] -
Vancouver
Yamamoto MM, Del Portillo HA. Luminescence-based assay for antimalarial drug screening. Resumos. 2005 ;[citado 2026 jan. 10 ] - Mining the malaria transcriptome
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