The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line (2005)
- Authors:
- Autor USP: JAEGER, RUY GASTALDONI - ICB
- Unidade: ICB
- Assunto: HISTOLOGIA
- Language: Português
- Abstract: Adenoid cystic carcinoma is a frequent malignant salivary gland neoplasm with high level of recurrence and distant metastasis. We have demonstrated that laminin-1 modulates the phenotype of an adenoid cystic carcinoma (CAC2) cell line. We are currently studying the expression of a non-integrin laminin receptor, the 32/67kDa laminin-binding protein (LBP), in adenoid cystic carcinoma. Immunohistochemistry showed the presence of LBP in adenoid cystic carcinoma in vivo. Immunofluorescence of CAC2 cells also revealed this receptor. The LBP binds to a sequence of the laminin beta 1 chain, the YIGSR peptide. This peptide promotes cell attachment and migration. Thus, we decided to study the role played by the peptide YIGSR and its ligand, the LBP receptor, in CAC2 cells. These cells were grown inside either laminin-1 (controls) or laminin-1 enriched with YIGSR (treated). Light microscopy showed that laminin-1 induced CAC2 cells to create solid formations of cells closely packed. On the other hand, CAC2 cells grown in contact with YIGSR showed a completely different pattern. Cells were non-cohesive and spindle shaped. To address whether the effect of YIGSR was dependent on LBP, CAC2 cells were pretreated with antibody against this receptor. Blockage of LBP receptor inhibited YIGSR effect on CAC2 morphology. We then decided to study whether YIGSR would inhibit the presence of adhesion molecules in CAC2 cells. We prepared cytoskeletal fractions of CAC2 cells grown eitherinside laminin-1 or laminin-1 enriched with YIGSR. Western blot of these fractions demonstrated that YIGSR decreased the amount of E-cadherin and beta-catenin in CAC2 cells, when compared to cells grown inside laminin-1. Pretreatment of CAC2 cells wit anti-LBP restored e-cadherin and beta catenin expression Conclusões: YIGSR and its ligand, 32/67kDa LBP regulates the expression of e-cadherin and beta-catenin in an adenoid cystic carcinoma cell line
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
FREITAS, V. M. et al. The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 07 fev. 2026. -
APA
Freitas, V. M., Oliveira, E., Furuse, C., Araújo, V. C., & Jaeger, R. G. (2005). The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Freitas VM, Oliveira E, Furuse C, Araújo VC, Jaeger RG. The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. Resumos. 2005 ;[citado 2026 fev. 07 ] -
Vancouver
Freitas VM, Oliveira E, Furuse C, Araújo VC, Jaeger RG. The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line. Resumos. 2005 ;[citado 2026 fev. 07 ] - Laminin-derived peptide AG73 regulates migration, invasion and protease activity of human oral squamous cell carcinoma cells through syndecan-1 and beta1 integrin
- Aspectos morfologicos da placa dental de incisivos de ratos
- Expressão das metaloproteinases da matriz no carcinoma adenóide cístico
- Efeito da laminina e do seu peptídio bioativo SIKVAV, no fenótipo do mioepitelioma
- Morphology and microanalysis of enamel pearl
- Expressão das metaloproteinases da matriz no carcinoma adenóide cístico
- Estudo de celulas cultivadas do adenoma pleomorfico humano. Analise do fenotipo celular frente a acao de proteinas da matriz extracelular. Analise da distribuicao citoesqueletal do filamento intermediario vimentina
- Comparison between solid and cystic histological patters of ameloblastoma by dna index and 'AG'nor technique
- Laminin-1-enhances secretory activity of a human salivary gland adenoid cystic carcinoma cell line
- Efeito da laminina e de seus peptídeos bioativos SIKVAV e AG-73 no fenótipo de linhagem celular derivada de adenoma pleomórfico
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
