Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain (2005)
- Authors:
- Autor USP: ZUCCHI, TANIA MARIA ARAUJO DOMINGUES - ICB
- Unidade: ICB
- Assunto: PARASITOLOGIA
- Language: Português
- Abstract: Objetivo: Total RNA from LPS stimulated rat macrophages was used to treat an Aspergillus nidulans (Emericella nidulans) strain resulting in the RT2 which culture supernatant showed anti-inflammatory activity in male Wistar rats (Cell Mol Biol. 42(2):235-40, 1996). Métodos e Resultados: The proteic fraction presenting such anti-inflammatory activity was purified and biochemically identified. The selection of the proteic fraction presenting the anti-inflammatory property was performed through the observation of podal oedema inhibition after carragenin induction, in male Swiss mice. Biochemical analyses, the method that presented the best results for peptide purification was that of molecular exclusion chromatography, since the peptide didn't adsorbed to other used chromatographic matrix. The total sugar quantification revealed that the peptide presented 32% of glycosilation. Relative to the pharmacological activity of this peptide, the oedema reduction occurred just in the first hour after oedema induction. The amino acid analyses showed a peculiar pattern with 29%of valine. The mass spectrometry revealed that the proteic sample is a conjugated glycopeptide with 5 peptides with 2.3 to 4.6 KDa. Conclusões: This work show, in first time, an anti-inflammatory activity on a supernatant glycopeptide never found before, but the biochemistry characterization of this sample
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
QUEIROZ, J. C. F. e TEIXEIRA, C. F. P. e ZUCCHI, Tânia Maria Araújo Domingues. Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 21 jan. 2026. -
APA
Queiroz, J. C. F., Teixeira, C. F. P., & Zucchi, T. M. A. D. (2005). Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Queiroz JCF, Teixeira CFP, Zucchi TMAD. Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. Resumos. 2005 ;[citado 2026 jan. 21 ] -
Vancouver
Queiroz JCF, Teixeira CFP, Zucchi TMAD. Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. Resumos. 2005 ;[citado 2026 jan. 21 ] - Análises genéticas da instabilidade mitótica de retrotrans-formantes de Aspergillus nidulans expressando homólogo da insulina humana
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- Efeito GIS (gene inactivations system) em linhagens de Aspergillus nidulans portadoras da duplicacao Dp (II, I)
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- Estudos de fatores genéticos que afetam a recombinação em regiões específicas do genoma do aspergillus nidulans
- Ribonucleic acid treatment alters gene expression in diploid strains of Aspergillus nidulans
- Estudo dos genes responsáveis pela regulação da expressão de fatores insulina -like de Aspergillus nidullans
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