Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain (2005)
- Authors:
- Autor USP: ZUCCHI, TANIA MARIA ARAUJO DOMINGUES - ICB
- Unidade: ICB
- Assunto: PARASITOLOGIA
- Language: Português
- Abstract: Objetivo: Total RNA from LPS stimulated rat macrophages was used to treat an Aspergillus nidulans (Emericella nidulans) strain resulting in the RT2 which culture supernatant showed anti-inflammatory activity in male Wistar rats (Cell Mol Biol. 42(2):235-40, 1996). Métodos e Resultados: The proteic fraction presenting such anti-inflammatory activity was purified and biochemically identified. The selection of the proteic fraction presenting the anti-inflammatory property was performed through the observation of podal oedema inhibition after carragenin induction, in male Swiss mice. Biochemical analyses, the method that presented the best results for peptide purification was that of molecular exclusion chromatography, since the peptide didn't adsorbed to other used chromatographic matrix. The total sugar quantification revealed that the peptide presented 32% of glycosilation. Relative to the pharmacological activity of this peptide, the oedema reduction occurred just in the first hour after oedema induction. The amino acid analyses showed a peculiar pattern with 29%of valine. The mass spectrometry revealed that the proteic sample is a conjugated glycopeptide with 5 peptides with 2.3 to 4.6 KDa. Conclusões: This work show, in first time, an anti-inflammatory activity on a supernatant glycopeptide never found before, but the biochemistry characterization of this sample
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
-
ABNT
QUEIROZ, J. C. F. e TEIXEIRA, C. F. P. e ZUCCHI, Tânia Maria Araújo Domingues. Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 14 maio 2024. -
APA
Queiroz, J. C. F., Teixeira, C. F. P., & Zucchi, T. M. A. D. (2005). Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Queiroz JCF, Teixeira CFP, Zucchi TMAD. Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. Resumos. 2005 ;[citado 2024 maio 14 ] -
Vancouver
Queiroz JCF, Teixeira CFP, Zucchi TMAD. Searching for a glycopeptidic antiinflamatory from RNA-treated aspergillus nidulans strain. Resumos. 2005 ;[citado 2024 maio 14 ] - Isolamento de mutantes mitocondriais que apresentam alterações nos padrões de recombinação, de reparo e/ou de metilação do DNA de Aspergillus nidulans
- Regulation of sterigmatocystin expression levels in Aspergillus nidulans strains of industrial interest
- Efeito da hemolinfa de insetos no padrão de metilação de genes marcadores de Aspergillus nidulans
- Estudo da indução de recombinação mitótica em Aspergillus nidulans sob condições de estresse térmico
- Preparation of protoplasts from the entomopathogenic fungus zoophthora radicans (zygomycetes, entomophthorales)
- RNA-treatment alters gene expression in diploid strains of Aspergillus nidulans
- Sterigmatocystin production by diploid strains of Aspergillus nidulans
- RNA-mediated transformation in Aspergillus nidulans recovers gene functions lost by deletion or by point mutations
- Determinacao da mutacao acr 'A IND.1' na duplicacao Dp (II-I) e do rearranjo induzivel pre-meioticamente (rip) em estudos de genetica classica efetuados em Aspergillus nidulans
- Estabilização da dup (ii-i), em mutantes de Aspergillus nidulans, através de processos genéticos
How to cite
A citação é gerada automaticamente e pode não estar totalmente de acordo com as normas
