Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes (2005)
- Authors:
- Autor USP: CHAVES, MARIA LUIZA MORAIS BARRETO DE - ICB
- Unidade: ICB
- Assunto: ANATOMIA
- Language: Português
- Abstract: Objetivo: Renin-Angiotensin System (RAS) has been associated with cardiac hypertrophy induced by thyroid hormone. However, few is known regarding the regulation of Angiotensin receptors in cardiac cells after hyperthyroidism condition. Métodos e Resultados Newborn rat ventricular cardiomyocytes were obtained from 1 to 2 day old Wistar rats. After successive digestion of tissue, cardiomyocytes were separated using a Percoll gradient and pooled in DMEM enriched with Newborn Calf and Horse Serum. 24 hours before the treatment, cells were maintained in serum free media. Cells were treated with crescent doses of T3 (1.M - 1?M) during 24 hours. After that, total RNA was obtained from cardiomyocytes and the ANF and AT2 gene expression were evaluated using semiquantitative RT-PCR. The ANF has been used for literature as a reliable cardiac hypertrophy marker. After treatment, cardiomyocytes presented a significant increase on ANF expression (up to 76%) showing the hypertrophic state of these cells. However, the AT2 expression was significantly decreased after treatment with T3, characterizing a doseresponse relation, which showed the lowest expression level with 0.1?M of T3 (21.43%). Conclusões: The effect of Thyroid hormone on decreasing AT2 receptors levels on cardiac myocytes suggests an important role of hormone on local/cardiac RAS regulation
- Imprenta:
- Publisher: Federação de Sociedades de Biologia Experimental
- Publisher place: Águas de Lindóia, São Paulo
- Date published: 2005
- Source:
- Título do periódico: Resumos
- Conference titles: Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE
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ABNT
RAMOS, M. S. C. e DINIZ, Gabriela Placoná e BARRETO-CHAVES, Maria Luiza Morais. Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. 2005, Anais.. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005. . Acesso em: 28 mar. 2024. -
APA
Ramos, M. S. C., Diniz, G. P., & Barreto-Chaves, M. L. M. (2005). Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. In Resumos. Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental. -
NLM
Ramos MSC, Diniz GP, Barreto-Chaves MLM. Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. Resumos. 2005 ;[citado 2024 mar. 28 ] -
Vancouver
Ramos MSC, Diniz GP, Barreto-Chaves MLM. Angiotensin type 2 receptor (AT2) expression in thyroid hormone-induced hypertrophy in primary cultured of cardiac myocytes. Resumos. 2005 ;[citado 2024 mar. 28 ] - Influence of thyroid hormone on the adenosine production in the cardiac tissue evaluated in subcellular fractions
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